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  Manipulation of β-carotene levels in tomato fruits results in increased ABA content and extended shelf-life

Diretto, G., Frusciante, S., Fabbri, C., Schauer, N., Busta, L., Wang, Z., et al. (in press). Manipulation of β-carotene levels in tomato fruits results in increased ABA content and extended shelf-life. Plant Biotechnology Journal, 0(ja). doi:10.1111/pbi.13283.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-3F15-E Version Permalink: http://hdl.handle.net/21.11116/0000-0005-3F17-C
Genre: Journal Article

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 Creators:
Diretto, Gianfranco1, Author
Frusciante, Sarah1, Author
Fabbri, Claudia1, Author
Schauer, N.2, Author              
Busta, Lucas1, Author
Wang, Zhonghua1, Author
Matas, Antonio J.1, Author
Fiore, Alessia1, Author
Rose, Jocelyn K.C.1, Author
Fernie, A. R.2, Author              
Jetter, Reinhard1, Author
Mattei, Benedetta1, Author
Giovannoni, Jim1, Author
Giuliano, Giovanni1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: tomato, β-carotene, ABA, ripening
 Abstract: Abstract Tomato fruit ripening is controlled by the hormone ethylene and by a group of transcription factors, acting upstream of ethylene. During ripening, the linear carotene lycopene accumulates at the expense of cyclic carotenoids. Fruit-specific overexpression of LYCOPENE β-CYCLASE (LCYb) resulted in increased β-carotene (provitamin A) content. Unexpectedly, LCYb-overexpressing fruits also exhibited a diverse array of ripening phenotypes, including delayed softening and extended shelf life. These phenotypes were accompanied, at the biochemical level, by an increase of abscisic acid (ABA) content, decreased ethylene production, increased density of cell wall material containing linear pectins with a low degree of methylation, and a thicker cuticle with a higher content of cutin monomers and triterpenoids. The levels of several primary metabolites and phenylpropanoid compounds were also altered in the transgenic fruits, which could be attributed to delayed fruit ripening and/or to ABA. Network correlation analysis and pharmacological experiments with the ABA biosynthesis inhibitor, abamine, indicated that altered ABA levels were a direct effect of the increased β-carotene content and were in turn responsible for the extended shelf life phenotype. Thus, manipulation of β-carotene levels results not only in an improvement of the nutritional value of tomato fruits, but also of their shelf life.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Accepted / In Press
 Pages: -
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 Rev. Method: -
 Identifiers: DOI: 10.1111/pbi.13283
BibTex Citekey: doi:10.1111/pbi.13283
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Title: Plant Biotechnology Journal
  Other : Plant Biotechnol. J.
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Pub.
Pages: - Volume / Issue: 0 (ja) Sequence Number: - Start / End Page: - Identifier: ISSN: 1467-7644
CoNE: https://pure.mpg.de/cone/journals/resource/110978984569611