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  An efficient method for the plating of haploid and diploid Emiliania huxleyi on solid medium

Skeffington, A. W., Grimm, A., Schönefeld, S., Petersen, K., & Scheffel, A. (2020). An efficient method for the plating of haploid and diploid Emiliania huxleyi on solid medium. Journal of Phycology, 56(1), 238-242. doi:10.1111/jpy.12942.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-3EFE-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-A911-9
Genre: Journal Article

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Skeffington, A. W.1, Author              
Grimm, A.1, Author              
Schönefeld, S.1, Author              
Petersen, K.2, Author              
Scheffel, A.1, Author              
Affiliations:
1Algal Biomineralization, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753322              
2Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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Free keywords: Coccolithophore, Emiliania, plating, haploid, diploid, genetics, microalgae, isolation clonal strains
 Abstract: Abstract Emiliania huxleyi is a globally important coccolithophore and one of the most successful eukaryotic organisms in the modern oceans. Despite a large body of work on this organism, including the sequencing of its genome, the tools required for forward and reverse functional genetic studies are still undeveloped. Here we present an optimized method for the clonal isolation of E. huxleyi by plating on solid medium. We demonstrate the utility of this method for a variety of strains including haploid, calcifying-diploid, and non-calcifying diploid strains. We show that, in contrast to previous studies, no changes in cell ploidy status occur when the cells are plated. Our method will greatly aid attempts to elucidate the genetic basis of the remarkable physiology of E. huxleyi by forward and reverse genetic approaches.

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Language(s): eng - English
 Dates: 20192020
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1111/jpy.12942
BibTex Citekey: doi:10.1111/jpy.12942
 Degree: -

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Title: Journal of Phycology
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Pub.
Pages: - Volume / Issue: 56 (1) Sequence Number: - Start / End Page: 238 - 242 Identifier: ISSN: 0022-3646
CoNE: https://pure.mpg.de/cone/journals/resource/954925417985