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Zusammenfassung:
The Sucrose Non-fermenting-1-Related Protein Kinase 1 (SnRK1) is a highly conserved heterotrimeric protein kinase in plants. It possesses a catalytic subunit (α) and two regulatory subunits (β and γ). The effects of altered expression of AKINβ1 on carbohydrate metabolism and gene expression in leaves were investigated in an Arabidopsis T-DNA insertion mutant. The contents of key intermediates in the tricarboxylic acid (TCA) cycle of the mutant leaves were markedly reduced throughout the diurnal cycle, coupled with a decrease in measurable respiration rate. Compared to wild-type, 2485 genes and 188 genes were expressed differentially in leaves of the akinβ1 mutant in response to light and darkness respectively. Among these, several genes exhibited very substantial decreases in expression. Notably, expression of particular isoforms of multigene families involved in malate and lipid metabolism, and nitrate uptake showed decreases of 40-240 fold during the light period, but not during darkness. The subcellular localization of AKINβ1 and the regulatory function of N-myristoylation on the subcellular localization of AKINβ1 were investigated, showing that AKINβ1 localizes to Golgi. A model is hypothesized to explain the effects of AKINβ1 on metabolism and gene expression in Arabidopsis.