English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  AKINβ1, a regulatory subunit of SnRK1, regulates organic acid metabolism and acts as a global regulator of genes involved in carbon, lipid and nitrogen metabolism

Wang, Y., Wang, L., Micallef, B. J., Tetlow, I. J., Mullen, R. T., Feil, R., et al. (2020). AKINβ1, a regulatory subunit of SnRK1, regulates organic acid metabolism and acts as a global regulator of genes involved in carbon, lipid and nitrogen metabolism. Journal of Experimental Botany, 71(3), 1010-1028. doi:10.1093/jxb/erz460.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-3EF4-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-8CE0-0
Genre: Journal Article

Files

show Files

Locators

show
hide
Locator:
Link (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Wang, You1, Author
Wang, Liping1, Author
Micallef, Barry J1, Author
Tetlow, Ian J1, Author
Mullen, Robert T1, Author
Feil, R.2, Author              
Lunn, J. E.2, Author              
Emes, Michael J1, Author
Affiliations:
1External Organizations, ou_persistent22              
2System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              

Content

show
hide
Free keywords: -
 Abstract: The Sucrose Non-fermenting-1-Related Protein Kinase 1 (SnRK1) is a highly conserved heterotrimeric protein kinase in plants. It possesses a catalytic subunit (α) and two regulatory subunits (β and γ). The effects of altered expression of AKINβ1 on carbohydrate metabolism and gene expression in leaves were investigated in an Arabidopsis T-DNA insertion mutant. The contents of key intermediates in the tricarboxylic acid (TCA) cycle of the mutant leaves were markedly reduced throughout the diurnal cycle, coupled with a decrease in measurable respiration rate. Compared to wild-type, 2485 genes and 188 genes were expressed differentially in leaves of the akinβ1 mutant in response to light and darkness respectively. Among these, several genes exhibited very substantial decreases in expression. Notably, expression of particular isoforms of multigene families involved in malate and lipid metabolism, and nitrate uptake showed decreases of 40-240 fold during the light period, but not during darkness. The subcellular localization of AKINβ1 and the regulatory function of N-myristoylation on the subcellular localization of AKINβ1 were investigated, showing that AKINβ1 localizes to Golgi. A model is hypothesized to explain the effects of AKINβ1 on metabolism and gene expression in Arabidopsis.

Details

show
hide
Language(s): eng - English
 Dates: 2019-102020
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1093/jxb/erz460
BibTex Citekey: 10.1093/jxb/erz460
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Experimental Botany
  Other : J. Exp. Bot
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford : Oxford University Press [etc.]
Pages: - Volume / Issue: 71 (3) Sequence Number: - Start / End Page: 1010 - 1028 Identifier: ISSN: 0022-0957
CoNE: https://pure.mpg.de/cone/journals/resource/954925413883