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  ATP synthesis and generation of electrochemical gradients of protons in the catecholamine storage organelle of the adrenal medulla

Taugner, G., & Wunderlich, I. (1981). ATP synthesis and generation of electrochemical gradients of protons in the catecholamine storage organelle of the adrenal medulla. Zeitschrift für Naturforschung, C: Journal of Biosciences, 36(11-12), 1056-1061. doi:10.1515/znc-1981-11-1225.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-F8B7-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-F8B8-5
Genre: Journal Article

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ZsNaturforschC_36_1981_1056.pdf (Any fulltext), 6MB
 
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 Creators:
Taugner, G.1, Author              
Wunderlich, Ilse2, Author              
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1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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Free keywords: Catecholamine Storage Organelles; Membrane Ghosts; Mg-AD P Evoked ΔμH·; ATP Synthesis; ATP-Pi Exchange
 Abstract: The reaction rates of ATP formation from ADP and inorganic phosphate (Pi) by the catecholamine storage organelles isolated from bovine adrenal medulla accelerated 5--6 fold, when Pi was added at various times after addition of Mg-ADP, as compared to the rates observed when the reaction partners were added simultaneously. The increase of the rates of ATP-Pi exchange upon subsequent addition of Pi to Mg-ATP was less prominent. Mg-ADP induced a delta psi (60--70 mV, positive inside), almost equal that induced by Mg-ATP. In both cases delta psi was significantly higher in the absence of Pi than in its presence. At pH 7.4 of the medium the delta pH was 1.4 units indicating an internal pH of 6. It was stable during the reaction time in the presence of Mg-ATP, while in the presence of Mg-ADP the internal pH increased gradually by 0.2 units. Both the ATP forming reactions as well as the nucleotide induced delta psi were uncoupler-sensitive (CCCP). Though inhibiting ATP formation from ADP + Pi by 50%, 0.1 mM AP5A (an inhibitor of adenylate kinase) did neither affect delta psi nor delta pH, hence ruling out the possibility that delta microH. induced by Mg-ADP would be actually due to AtP formed from ADP. Membrane ghosts were not able of ATP synthesis (in the absence of valinomycin), the ATP-Pi exchange was only 10--20% of that of intact organelles, due to the low delta microH. in the absence of intravesicular soluble constituents. It is proposed that the energy for ATP synthesis is furnished by the high gradients of protonated soluble constituents in the intact organelles.

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Language(s): eng - English
 Dates: 1981-08-172014-06-021981-12-01
 Publication Status: Published in print
 Pages: 6
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 Rev. Type: Peer
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Title: Zeitschrift für Naturforschung, C: Journal of Biosciences
  Abbreviation : Z. Naturforsch., C: J. Biosci.
Source Genre: Journal
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Publ. Info: Berlin : Walter de Gruyter GmbH
Pages: - Volume / Issue: 36 (11-12) Sequence Number: - Start / End Page: 1056 - 1061 Identifier: ISSN: 1865-7125
CoNE: https://pure.mpg.de/cone/journals/resource/954927655916_1