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  Metabolic profiling of Saccharomyces paradoxus using FT-ICR-MS

Reinhold, A. (2019). Metabolic profiling of Saccharomyces paradoxus using FT-ICR-MS. Master Thesis, Christian-Albrechts-Universität zu Kiel, Kiel.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-09AF-D Version Permalink: http://hdl.handle.net/21.11116/0000-0005-1D2D-A
Genre: Thesis

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 Creators:
Reinhold, Antonia, Author
Schwarz, Karin, Referee
Stukenbrock, Eva H.1, Referee              
Affiliations:
1Max Planck Fellow Group Environmental Genomics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2068284              

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 Abstract: In this thesis, the viability of the yeast species Saccharomyces paradoxus was investigated by examining the metabolic profile of energy metabolism in their natural habitat using FTICR- MS. For this purpose, different yeast strains of the species Saccharomyces paradoxus were cultivated in the yeast medium, which was collected from their natural habitat at five different seasons and two different substrates (soil and leaf litter) and compared at three timepoints after yeast cell addition (T0, T05, T24). The results of the work may partially confirm the findings that glycolysis and the citrate cycle are the main energy metabolism of yeasts. In addition, new insights are given that allow the use of alternative energy sources by Saccharomyces paradoxus and the course of metabolic pathways, enabling them to survive in nature in the absence of sugars. In the PCA models, differentiations were observed at time T05 on the one hand and in the seasons on the other, with cluster formation at November 2017. Through the extracellular examination of the metabolite spectrum of Saccharomyces paradoxus, the use of alternative substances as energy sources, the connections and functions to each other and to the energy metabolism could be partially elucidated. The energy metabolism of yeasts is very diverse, especially when using compounds that can serve as energy sources. Nevertheless, within the scope of this work, slight insights into these have been made possible. Since glycolysis is described as one of the main energy metabolisms of yeasts, it is surprising that only two metabolites, acetaldehyde and lactate, could be identified. However, this can be attributed to the presence of furfural, which has an inhibitory effect on glycolysis. The detection of lactate can be traced back to a possible self-synthesis by yeasts, which occurs independently of the substrate. Also, a conversion of lactaldehyde or the reaction of acetaldehyde can lead to lactate production. Lactate can then serve as a precursor of glycerol, which is then used as an energy source. The identification of cis-aconitate, succinate and isocitrate or citrate suggests that Saccharomyces paradoxus operates the citrate cycle as an energy metabolism. However, these compounds also occur in the glyoxylate bypass, which enables the growth of microorganisms in the absence of sugars. Yeasts mainly produce glycerol as a by-product of sugar metabolism and can be regulated by various nutritional and environmental factors such as temperature, osmosis and nitrogen source. Through de novo synthesis, yeasts can synthesize glycerol themselves, which they use and degrade under aerobic conditions as a source of energy and carbon. The presence 98 of DMSO can also lead to glycerol production, since yeasts form balancing substances such as glycerol to protect cells against oxidative stress. Nitrogen sources are required for yeast growth and TCA, influence glycerol production in yeast cells and can promote lipid production, which yeast can then use as an energy source. Urea could serve as a nitrogen source, release toxic compounds from yeast cells or increase fatty acid production. The degradation of creatinine may suggest that this compound is used as an energy source by Saccharomyces paradoxus. The function of fatty acids such as lauric acid, palmitic acid and stearic acid is that they can be used for membrane synthesis, prostaglandin formation and as a source of energy through Saccharomyces paradoxus. Likewise, the influence of growth temperature can have an effect on the presence of fatty acids in the culture medium, so that the behaviour and presence of fatty acids cannot be generalized.

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Language(s): eng - English
 Dates: 2019-032019-03
 Publication Status: Published in print
 Pages: 138
 Publishing info: Kiel : Christian-Albrechts-Universität zu Kiel
 Table of Contents: -
 Rev. Method: -
 Identifiers: Other: Dipl
 Degree: Master

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