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  Thermodynamic control of −1 programmed ribosomal frameshifting.

Bock, L. V., Caliskan, N., Korniy, N., Peske, F., Rodnina, M. V., & Grubmüller, H. (2019). Thermodynamic control of −1 programmed ribosomal frameshifting. Nature Communications, 10: 4598. doi:10.1038/s41467-019-12648-x.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-0A90-D Version Permalink: http://hdl.handle.net/21.11116/0000-0005-4533-4
Genre: Journal Article

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 Creators:
Bock, L. V.1, Author              
Caliskan, N.2, Author              
Korniy, N.2, Author              
Peske, F.2, Author              
Rodnina, M. V.2, Author              
Grubmüller, H.1, Author              
Affiliations:
1Department of Theoretical and Computational Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578631              
2Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              

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 Abstract: mRNA contexts containing a ‘slippery’ sequence and a downstream secondary structure element stall the progression of the ribosome along the mRNA and induce its movement into the −1 reading frame. In this study we build a thermodynamic model based on Bayesian statistics to explain how −1 programmed ribosome frameshifting can work. As training sets for the model, we measured frameshifting efficiencies on 64 dnaX mRNA sequence variants in vitro and also used 21 published in vivo efficiencies. With the obtained free-energy difference between mRNA-tRNA base pairs in the 0 and −1 frames, the frameshifting efficiency of a given sequence can be reproduced and predicted from the tRNA−mRNA base pairing in the two frames. Our results further explain how modifications in the tRNA anticodon modulate frameshifting and show how the ribosome tunes the strength of the base-pair interactions.

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Language(s): eng - English
 Dates: 2019-10-10
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41467-019-12648-x
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Title: Nature Communications
Source Genre: Journal
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Pages: 11 Volume / Issue: 10 Sequence Number: 4598 Start / End Page: - Identifier: -