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  Multiphoton Ca2+ imaging of astrocytes with genetically encoded indicators delivered by a viral approach

Enger, R., Sprengel, R., Nagelhus, E. A., & Tang, W. (2019). Multiphoton Ca2+ imaging of astrocytes with genetically encoded indicators delivered by a viral approach. Multiphoton Microscopy, 251-277. doi:10.1007/978-1-4939-9702-2_11.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-0BFA-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-0BFB-5
Genre: Journal Article

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 Creators:
Enger, Rune, Author
Sprengel, Rolf1, 2, 3, Author              
Nagelhus, Erlend A.1, Author              
Tang, Wannan, Author
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Olfaction Web, Max Planck Institute for Medical Research, Max Planck Society, ou_1497733              
3Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497741              

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Free keywords: Astrocytes; Ca2+ signaling; Genetically encoded calcium indicator; GECI; GCaMP6; Glia; rAAV gene transduction; Two-photon microscopy
 Abstract: Decades of research have unraveled the complex functioning of neurons in the central nervous system. Our knowledge of the second main player of the brain – the non-excitable glial cells – clearly lags behind that of neurons. Pioneering work in the 1990s provided evidence that star-shaped glial cells – astrocytes – sense and modulate neuronal activity by intracellular Ca2+ signals. However, the precise roles of astrocytic Ca2+ signaling in brain physiology and pathophysiology are still highly controversial, largely due to technical limitations of previous Ca2+ imaging tools. With recent innovations in laser microscopy and engineering of molecular probes, the field of glioscience is undergoing a revolution. This chapter describes the application of multiphoton microscopy and genetically encoded fluorescent Ca2+ indicators to reveal astrocytic Ca2+ signals in acute brain slices and in vivo, both in anesthetized and in awake behaving animals.

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Language(s): eng - English
 Dates: 2019-11-012019-11-01
 Publication Status: Published in print
 Pages: 27
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1007/978-1-4939-9702-2_11
 Degree: -

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Title: Multiphoton Microscopy
Source Genre: Book
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Publ. Info: Humana, New York, NY : Springer
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 251 - 277 Identifier: ISBN: 978-1-4939-9701-5
ISBN: 978-1-4939-9702-2