English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Current Source Density Profiles of Optical Recording Maps: a New Approach to the Analysis of Spatio‐temporal Neural Activity Patterns

Plenz, D., & Aertsen, A. (1993). Current Source Density Profiles of Optical Recording Maps: a New Approach to the Analysis of Spatio‐temporal Neural Activity Patterns. European Journal of Neuroscience: European Neuroscience Association, 5(5), 437-445. doi:10.1111/j.1460-9568.1993.tb00510.x.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-0C6D-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-0C6E-4
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Plenz, D1, 2, Author              
Aertsen, A1, 2, Author              
Affiliations:
1Former Department Structure and Function of Natural Nerve-Net , Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497803              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              

Content

show
hide
Free keywords: -
 Abstract: patio‐temporal spreading of activity in the CA1 region of the rat hippocampal slice was studied by two experimental approaches. At identical locations in the tissue we measured both the extracellular field potential distribution with microelectrode recordings and the intracellular potential distribution by optical recording, using voltage‐sensitive fluorescent dyes. Current source density analysis (CSD) was applied to the extracellular field potential distributions (eCSD) to enhance the spatial resolution. In order to obtain an analogous improvement for the optical recordings, we developed a new CSD transformation, which calculates the locations of the transmembrane current generators from the intracellular potential distributions (iCSD). Compared to the underlying fluorescence maps, the new iCSD profiles exhibit a considerable improvement in spatial resolution. Results can be directly interpreted in terms of physiological membrane processes, such as postsynaptic potentials and action potentials. The iCSD profiles show a surprisingly good correspondence with the classical eCSD profiles both qualitatively and quantitatively, the only difference being that cell body activity is reduced in amplitude. Thus, this new optical CSD analysis paves the way for a quantitative interpretation, rather than the hitherto predominantly qualitative interpretation of spatio‐temporal activity profiles from optical recording measurements.

Details

show
hide
Language(s):
 Dates: 1993-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: European Journal of Neuroscience : European Neuroscience Association
  Other : Eur. J. Neurosci
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford, UK : Published on behalf of the European Neuroscience Association by Oxford University Press
Pages: - Volume / Issue: 5 (5) Sequence Number: - Start / End Page: 437 - 445 Identifier: ISSN: 0953-816X
CoNE: https://pure.mpg.de/cone/journals/resource/954925575988