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  Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder

Alagesan, K., & Kolarich, D. (2019). Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder. MethodsX, 6, 773-778. doi:10.1016/j.mex.2019.04.007.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-1676-E Version Permalink: http://hdl.handle.net/21.11116/0000-0005-1677-D
Genre: Journal Article

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 Creators:
Alagesan, Kathirvel1, Author              
Kolarich, Daniel1, Author              
Affiliations:
1Daniel Kolarich, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863301              

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Free keywords: Improved strategy for large scale isolation and purification of sialylglycopeptide from egg yolk powder, Synthetic glycobiology, Glycopeptide isolation, Egg yolk powder
 Abstract: Chicken egg yolk is an easily available source for the isolation of sialylglycopeptides (SGP) carrying homogenous biantennary N-glycans. This approach has gained much attention in the last decade since these SGPs can easily be used for the semi-synthesis of glycoconjugates circumventing laborious full-synthetic methodologies. Here we report an optimised, significantly shorter (one day instead of five) and environmentally friendly procedure for the mg scale isolation of SGP using commercially available egg yolk powder. A single chromatographic step following chloroform/methanol precipitation of proteins and lipids yielded desired approximately 200 mg SGP from 250 g egg yolk powder within a day. •Environmentally friendly procedure for isolation of sialylglycopeptide from Egg yolk powder.•Reduced the protocol from five days down to one.

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Language(s): eng - English
 Dates: 2019-04-092019
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1016/j.mex.2019.04.007
BibTex Citekey: ALAGESAN2019773
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Title: MethodsX
  Abbreviation : MethodsX
Source Genre: Journal
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Publ. Info: Amsterdam, The Netherlands : Elsevier BV
Pages: - Volume / Issue: 6 Sequence Number: - Start / End Page: 773 - 778 Identifier: ISSN: 2215-0161