English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A comparative study of single-particle cryo-EM with liquid-nitrogen and liquid-helium cooling

Pfeil-Gardiner, O., Mills, D. J., Vonck, J., & Kuehlbrandt, W. (2019). A comparative study of single-particle cryo-EM with liquid-nitrogen and liquid-helium cooling. IUCrJ, 6(Pt 6), 1099-1105. doi:10.1107/S2052252519011503.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-1A3C-C Version Permalink: http://hdl.handle.net/21.11116/0000-0005-1A3D-B
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Pfeil-Gardiner, Olivia1, Author              
Mills, Deryck J.1, Author              
Vonck, Janet1, Author              
Kuehlbrandt, Werner1, Author              
Affiliations:
1Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              

Content

show
hide
Free keywords: apoferritin, beam-induced motion, beam-induced movement, cryo-EM, electron cryo-microscopy, helium, liquid-helium cooling, radiation damage
 Abstract: Radiation damage is the most fundamental limitation for achieving high resolution in electron cryo-microscopy (cryo-EM) of biological samples. The effects of radiation damage are reduced by liquid-helium cooling, although the use of liquid helium is more challenging than that of liquid nitrogen. To date, the benefits of liquid-nitrogen and liquid-helium cooling for single-particle cryo-EM have not been compared quantitatively. With recent technical and computational advances in cryo-EM image recording and processing, such a comparison now seems timely. This study aims to evaluate the relative merits of liquid-helium cooling in present-day single-particle analysis, taking advantage of direct electron detectors. Two data sets for recombinant mouse heavy-chain apoferritin cooled with liquid-nitrogen or liquid-helium to 85 or 17 K were collected, processed and compared. No improvement in terms of resolution or Coulomb potential map quality was found for liquid-helium cooling. Interestingly, beam-induced motion was found to be significantly higher with liquid-helium cooling, especially within the most valuable first few frames of an exposure, thus counteracting any potential benefit of better cryoprotection that liquid-helium cooling may offer for single-particle cryo-EM.

Details

show
hide
Language(s): eng - English
 Dates: 2019-05-142019-08-162019-11
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1107/S2052252519011503
BibTex Citekey: pfeil-gardiner_comparative_2019
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: IUCrJ
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Chester CH1 2HU, England : International Union of Crystallography (IUCr)
Pages: - Volume / Issue: 6 (Pt 6) Sequence Number: - Start / End Page: 1099 - 1105 Identifier: ISSN: 2052-2525
CoNE: https://pure.mpg.de/cone/journals/resource/2052-2525