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  Vimentin intermediate filaments undergo irreversible conformational changes during cyclic loading

Forsting, J., Kraxner, J., Witt, H., Janshoff], A., & Köster, S. (2019). Vimentin intermediate filaments undergo irreversible conformational changes during cyclic loading. Nano Letters, 19(10), 7349-7356. doi:10.1021/acs.nanolett.9b02972.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-17FB-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-17FC-6
Genre: Journal Article

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 Creators:
Forsting, J., Author
Kraxner , J., Author
Witt, Hannes1, Author              
Janshoff], A., Author
Köster , S., Author
Affiliations:
1Max Planck Fellow Group Membrane-based biomimetic nano- and micro-compartments, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2586691              

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Free keywords: cell mechanics; cytoskeleton; intermediate filaments; force-strain behavior; 3-state system; optical tweezers
 Abstract: Intermediate filaments (IFs) are part of the cytoskeleton of eukaryotic cells and, therefore, are largely responsible for the cell's mechanical properties. IFs are characterized by a pronounced extensibility and remarkable resilience that enable them to support cells in extreme situations. Previous experiments showed that, under strain, alpha-helices in vimentin IFs might unfold to beta-sheets. Upon repeated stretching, the filaments soften; however, the remaining plastic strain is negligible. Here, we observe that vimentin IFs do not recover their original stiffness on reasonable time scales, and we explain these seemingly contradicting results by introducing a third, less well-defined conformational state. Reversibility on the nanoscale can be fully rescued by introducing cross-linkers that prevent transition to the beta-sheet. Our results classify IFs as a nanomaterial with intriguing mechanical properties, which is likely to play a major role for the cell's local adaption to external stimuli.

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Language(s): eng - English
 Dates: 2019-09-062019-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1021/acs.nanolett.9b02972
 Degree: -

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Title: Nano Letters
Source Genre: Journal
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Pages: - Volume / Issue: 19 (10) Sequence Number: - Start / End Page: 7349 - 7356 Identifier: -