English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Insights into the assembly and architecture of a Staufen-mediated mRNA decay (SMD)-competent mRNP.

Gowravaram, M., Schwarz, J., Khilji, S. K., Urlaub, H., & Chakrabarti, S. (2019). Insights into the assembly and architecture of a Staufen-mediated mRNA decay (SMD)-competent mRNP. Nature Communications, 10: 5054. doi:10.1038/s41467-019-13080-x.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-3E58-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-5F87-9
Genre: Journal Article

Files

show Files
hide Files
:
Article.pdf (Publisher version), 3MB
Name:
Article.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Gowravaram, Manjeera, Author
Schwarz, Juliane, Author
Khilji, Sana K.1, Author              
Urlaub, Henning, Author
Chakrabarti, Sutapa, Author
Affiliations:
1Oren Moscovitz, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_3176803              

Content

show
hide
Free keywords: -
 Abstract: The mammalian Staufen proteins (Stau1 and Stau2) mediate degradation of mRNA containing complex secondary structures in their 3'-untranslated region (UTR) through a pathway known as Staufen-mediated mRNA decay (SMD). This pathway also involves the RNA helicase UPF1, which is best known for its role in the nonsense-mediated mRNA decay (NMD) pathway. Here we present a biochemical reconstitution of the recruitment and activation of UPF1 in context of the SMD pathway. We demonstrate the involvement of UPF2, a core NMD factor and a known activator of UPF1, in SMD. UPF2 acts as an adaptor between Stau1 and UPF1, stimulates the catalytic activity of UPF1 and plays a central role in the formation of an SMD-competent mRNP. Our study elucidates the molecular mechanisms of SMD and points towards extensive cross-talk between UPF1-mediated mRNA decay pathways in cells.

Details

show
hide
Language(s): eng - English
 Dates: 2019-11-072019
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 31699982
DOI: 10.1038/s41467-019-13080-x
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 10 Sequence Number: 5054 Start / End Page: - Identifier: ISSN: 2041-1723