English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  In vivo optochemical control of cell contractility at single-cell resolution

Kong, D., Lv, Z., Häring, M., Lin, B., Wolf, F., & Grosshans, J. (2019). In vivo optochemical control of cell contractility at single-cell resolution. EMBO Reports, e47755. doi:10.15252/embr.201947755.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-206E-C Version Permalink: http://hdl.handle.net/21.11116/0000-0005-206F-B
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Kong, D., Author
Lv, Z., Author
Häring, Matthias1, Author              
Lin, B., Author
Wolf, Fred1, Author              
Grosshans, J., Author
Affiliations:
1Research Group Theoretical Neurophysics, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063289              

Content

show
hide
Free keywords: actomyosin; Ca2+ uncaging; cell contractility; morphogenesis; optochemical
 Abstract: The spatial and temporal dynamics of cell contractility plays a key role in tissue morphogenesis, wound healing, and cancer invasion. Here, we report a simple optochemical method to induce cell contractions in vivo during Drosophila morphogenesis at single-cell resolution. We employed the photolabile Ca2+ chelator o-nitrophenyl EGTA to induce bursts of intracellular free Ca2+ by laser photolysis in the epithelial tissue. Ca2+ bursts appear within seconds and are restricted to individual target cells. Cell contraction reliably followed within a minute, causing an approximately 50% drop in the cross-sectional area. Increased Ca2+ levels are reversible, and the target cells further participated in tissue morphogenesis. Depending on Rho kinase (ROCK) activity but not RhoGEF2, cell contractions are paralleled with non-muscle myosin II accumulation in the apico-medial cortex, indicating that Ca2+ bursts trigger non-muscle myosin II activation. Our approach can be, in principle, adapted to many experimental systems and species, as no specific genetic elements are required.

Details

show
hide
Language(s): eng - English
 Dates: 2019-10-02
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.15252/embr.201947755
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: EMBO Reports
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 14 Volume / Issue: - Sequence Number: e47755 Start / End Page: - Identifier: -