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  Assessment of young and aged hematopoietic stem cell activity by competitive serial transplantation assays

Zhang, Y. W., & Cabezas-Wallscheid, N. (2019). Assessment of young and aged hematopoietic stem cell activity by competitive serial transplantation assays. In Methods in Molecular Biology (pp. 193-203). Springer. doi:10.1007/978-1-4939-9574-5_15.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0005-3A3A-A Version Permalink: https://hdl.handle.net/21.11116/0000-0005-3A3B-9
Genre: Book Chapter

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 Creators:
Zhang, Yu Wei1, Author
Cabezas-Wallscheid, Nina1, Author           
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1Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, ou_2243641              

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Free keywords: Aged, Bone marrow, Competitive, Hematopoietic stem cells, Transplants, Young
 Abstract: Healthy hematopoietic stem cells (HSCs) are capable to self-renew and reconstitute the complete hematopoietic system. Upon aging, there is an increased incidence of blood-related diseases. Age-related phenotypes have been widely studied by bone marrow transplantation experiments, where reconstitution of the transplanted cells is a direct measure of HSC activity. In this protocol we describe a competitive bone marrow transplantation assay to functionally test young and old HSCs.

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Language(s): eng - English
 Dates: 2019-06-142019
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-4939-9574-5_15
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Title: Methods in Molecular Biology
Source Genre: Book
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Publ. Info: Springer
Pages: - Volume / Issue: 2017 Sequence Number: - Start / End Page: 193 - 203 Identifier: -