English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Proteomic comparison of malignant human germ cell tumor cell lines.

Bremmer, F., Bohnenberger, H., Küffer, S., Oellerich, T., Serve, H., Urlaub, H., et al. (2019). Proteomic comparison of malignant human germ cell tumor cell lines. Disease Markers, 2019: 8298524. doi:10.1155/2019/8298524.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-3AA6-F Version Permalink: http://hdl.handle.net/21.11116/0000-0005-3AAC-9
Genre: Journal Article

Files

show Files
hide Files
:
3177411.pdf (Publisher version), 7MB
Name:
3177411.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3177411_Suppl_1.xlsx (Supplementary material), 5MB
Name:
3177411_Suppl_1.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3177411_Suppl_2.xlsx (Supplementary material), 461KB
Name:
3177411_Suppl_2.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3177411_Suppl_3.xls (Supplementary material), 35KB
Name:
3177411_Suppl_3.xls
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.ms-excel / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Bremmer, F., Author
Bohnenberger, H., Author
Küffer, S., Author
Oellerich, T., Author
Serve, H., Author
Urlaub, H.1, Author              
Strauss, A., Author
Maatoug, Y., Author
Behnes, C. L., Author
Oing, C., Author
Radzun, H. J., Author
Ströbel, P., Author
Balabanov, S., Author
Honecker, F., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

Content

show
hide
Free keywords: -
 Abstract: Malignant germ cell tumors (GCT) are the most common malignant tumors in young men between 18 and 40 years. The correct identification of histological subtypes, in difficult cases supported by immunohistochemistry, is essential for therapeutic management. Furthermore, biomarkers may help to understand pathophysiological processes in these tumor types. Two GCT cell lines, TCam-2 with seminoma-like characteristics, and NTERA-2, an embryonal carcinoma-like cell line, were compared by a quantitative proteomic approach using high-resolution mass spectrometry (MS) in combination with stable isotope labelling by amino acid in cell culture (SILAC). We were able to identify 4856 proteins and quantify the expression of 3936. 347 were significantly differentially expressed between the two cell lines. For further validation, CD81, CBX-3, PHF6, and ENSA were analyzed by western blot analysis. The results confirmed the MS results. Immunohistochemical analysis on 59 formalin-fixed and paraffin-embedded (FFPE) normal and GCT tissue samples (normal testis, GCNIS, seminomas, and embryonal carcinomas) of these proteins demonstrated the ability to distinguish different GCT subtypes, especially seminomas and embryonal carcinomas. In addition, siRNA-mediated knockdown of these proteins resulted in an antiproliferative effect in TCam-2, NTERA-2, and an additional embryonal carcinoma-like cell line, NCCIT. In summary, this study represents a proteomic resource for the discrimination of malignant germ cell tumor subtypes and the observed antiproliferative effect after knockdown of selected proteins paves the way for the identification of new potential drug targets.

Details

show
hide
Language(s): eng - English
 Dates: 2019-09-03
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1155/2019/8298524
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Disease Markers
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 14 Volume / Issue: 2019 Sequence Number: 8298524 Start / End Page: - Identifier: -