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  Mass Spectrometry-Based Untargeted Plant Metabolomics

de Souza, L. P., Alseekh, S., Naake, T., & Fernie, A. R. (2019). Mass Spectrometry-Based Untargeted Plant Metabolomics. Current Protocols in Plant Biology, 4(4): e20100. doi:10.1002/cppb.20100.

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de Souza, L. P.1, Author              
Alseekh, S.1, Author              
Naake, T.1, Author              
Fernie, A. R.1, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: LC-MS, metabolomics, untargeted, plant metabolomics, metabolite annotation, molecular networking
 Abstract: Abstract Metabolomics has grown into one of the major approaches for systems biology studies, in part driven by developments in mass spectrometry (MS), providing high sensitivity and coverage of the metabolome at high throughput. Untargeted metabolomics allows for the investigation of metabolic phenotypes involving several hundreds to thousands of metabolites. In this approach, all signals in a mass chromatogram are processed in an unbiased way, allowing for a deeper investigation of metabolic phenotypes, but also resulting in significantly more complex data processing and post-processing steps. In this article, we discuss all the intricacies involved in extracting and analyzing metabolites by chromatography coupled to MS, as well as the processing and analysis of such datasets. © 2019 The Authors. Basic Protocol 1: Metabolite extraction for LC-MS Alternate Protocol: Methyl tert-butyl ether (MTBE) extraction for multiple mass spectrometry platforms (GC-polar, LC-polar, LC-lipid) Basic Protocol 2: LC-MS analysis Support Protocol 1: GC-MS derivatization and analysis Support Protocol 2: Lipid analysis Basic Protocol 3: LC-MS data processing Basic Protocol 4: Data analysis Basic Protocol 5: Metabolite annotation Support Protocol 3: Molecular networking using MetNet Support Protocol 4: Co-injection of authentic standards

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: DOI: 10.1002/cppb.20100
BibTex Citekey: doi:10.1002/cppb.20100
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Title: Current Protocols in Plant Biology
Source Genre: Journal
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Pages: - Volume / Issue: 4 (4) Sequence Number: e20100 Start / End Page: - Identifier: -