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  Radial somatic F‐actin organization affects growth cone dynamics during early neuronal development

Meka, D. P., Scharrenberg, R., Zhao, B., Kobler, O., König, T., Schaefer, I., et al. (2019). Radial somatic F‐actin organization affects growth cone dynamics during early neuronal development. EMBO Reports, 20(12): e47743. doi:10.15252/embr.201947743.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-4759-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-1006-1
Genre: Journal Article

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Meka_et_al-2019-EMBO_reports.pdf (Publisher version), 7MB
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ard_ard_2019_meka_embo-rep_20_11_e47743_suppl1.zip (Supplementary material), 14MB
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"Appendix" (pdf); "Expanded View Figures PDF" (pdf) | for "Movie EV1" - "Movie EV14" visit https://www.embopress.org/doi/10.15252/embr.201947743#support-information-section (14 zipped avi-files with docx-comments, altogether 271 MB)
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https://dx.doi.org/10.15252/embr.201947743 (Publisher version)
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https://dx.doi.org/10.1101/372813 (Preprint)
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 Creators:
Meka, D. P.1, Author
Scharrenberg, R.1, Author
Zhao, B.1, Author
Kobler, O.2, Author
König, T.1, Author
Schaefer, I.1, Author
Schwanke, B.1, Author
Klykov, S.3, Author
Richter, M.1, Author
Eggert, D.4, 5, Author              
Windhorst, S.6, Author
Dotti, C. G.7, Author
Kreutz, M. R.8, 9, Author
Mikhaylova, M.3, Author
de Anda, F. C.1, Author
Affiliations:
1RG Neuronal Development, Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg‐Eppendorf, ou_persistent22              
2Combinatorial Neuroimaging Core Facility (CNI), Leibniz Institute for Neurobiology, Magdeburg, ou_persistent22              
3Emmy‐Noether Group “Neuronal Protein Transport”, Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg‐Eppendorf, ou_persistent22              
4Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
5Heinrich Pette Institute—Leibniz Institute for Experimental Virology, Hamburg, ou_persistent22              
6Department of Biochemistry and Signal Transduction, University Medical Center Hamburg‐Eppendorf, ou_persistent22              
7Centro de Biología Molecular “Severo Ochoa”, CSIC‐UAM, Madrid, ou_persistent22              
8RG Neuroplasticity, Leibniz Institute for Neurobiology, Magdeburg, ou_persistent22              
9Leibniz Guest Group “Dendritic Organelles and Synaptic Function”, Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg‐Eppendorf, ou_persistent22              

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Free keywords: actin; centrosome; microtubules; neuronal development; PCM-1
 Abstract: The centrosome is thought to be the major neuronal microtubule‐organizing center (MTOC) in early neuronal development, producing microtubules with a radial organization. In addition, albeit in vitro, recent work showed that isolated centrosomes could serve as an actin‐organizing center, raising the possibility that neuronal development may, in addition, require a centrosome‐based actin radial organization. Here, we report, using super‐resolution microscopy and live‐cell imaging of cultured rodent neurons, F‐actin organization around the centrosome with dynamic F‐actin aster‐like structures with F‐actin fibers extending and retracting actively. Photoactivation/photoconversion experiments and molecular manipulations of F‐actin stability reveal a robust flux of somatic F‐actin toward the cell periphery. Finally, we show that somatic F‐actin intermingles with centrosomal PCM‐1 (pericentriolar material 1 protein) satellites. Knockdown of PCM‐1 and disruption of centrosomal activity not only affect F‐actin dynamics near the centrosome but also in distal growth cones. Collectively, the data show a radial F‐actin organization during early neuronal development, which might be a cellular mechanism for providing peripheral regions with a fast and continuous source of actin polymers, hence sustaining initial neuronal development.

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Language(s): eng - English
 Dates: 2019-09-062019-01-182019-09-272019-10-242019-12-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.15252/embr.201947743
 Degree: -

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Title: EMBO Reports
  Other : EMBO Rep.
Source Genre: Journal
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Publ. Info: Oxford, UK : Published for EMBO by Oxford University Press
Pages: - Volume / Issue: 20 (12) Sequence Number: e47743 Start / End Page: - Identifier: ISSN: 1469-221X
CoNE: https://pure.mpg.de/cone/journals/resource/110978984569661