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  Radial somatic F‐actin organization affects growth cone dynamics during early neuronal development

Meka, D. P., Scharrenberg, R., Zhao, B., Kobler, O., König, T., Schaefer, I., et al. (2019). Radial somatic F‐actin organization affects growth cone dynamics during early neuronal development. EMBO Reports, 20(12): e47743. doi:10.15252/embr.201947743.

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Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-0005-4759-8 Versions-Permalink: https://hdl.handle.net/21.11116/0000-000B-AE0E-3
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"Appendix" (pdf); "Expanded View Figures PDF" (pdf) | for "Movie EV1" - "Movie EV14" visit https://www.embopress.org/doi/10.15252/embr.201947743#support-information-section (14 zipped avi-files with docx-comments, altogether 271 MB)
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https://dx.doi.org/10.15252/embr.201947743 (Verlagsversion)
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 Urheber:
Meka, D. P.1, Autor
Scharrenberg, R.1, Autor
Zhao, B.1, Autor
Kobler, O.2, Autor
König, T.1, Autor
Schaefer, I.1, Autor
Schwanke, B.1, Autor
Klykov, S.3, Autor
Richter, M.1, Autor
Eggert, D.4, 5, Autor           
Windhorst, S.6, Autor
Dotti, C. G.7, Autor
Kreutz, M. R.8, 9, Autor
Mikhaylova, M.3, Autor
de Anda, F. C.1, Autor
Affiliations:
1RG Neuronal Development, Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg‐Eppendorf, ou_persistent22              
2Combinatorial Neuroimaging Core Facility (CNI), Leibniz Institute for Neurobiology, Magdeburg, ou_persistent22              
3Emmy‐Noether Group “Neuronal Protein Transport”, Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg‐Eppendorf, ou_persistent22              
4Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
5Heinrich Pette Institute—Leibniz Institute for Experimental Virology, Hamburg, ou_persistent22              
6Department of Biochemistry and Signal Transduction, University Medical Center Hamburg‐Eppendorf, ou_persistent22              
7Centro de Biología Molecular “Severo Ochoa”, CSIC‐UAM, Madrid, ou_persistent22              
8RG Neuroplasticity, Leibniz Institute for Neurobiology, Magdeburg, ou_persistent22              
9Leibniz Guest Group “Dendritic Organelles and Synaptic Function”, Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg‐Eppendorf, ou_persistent22              

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Schlagwörter: actin; centrosome; microtubules; neuronal development; PCM-1
 Zusammenfassung: The centrosome is thought to be the major neuronal microtubule‐organizing center (MTOC) in early neuronal development, producing microtubules with a radial organization. In addition, albeit in vitro, recent work showed that isolated centrosomes could serve as an actin‐organizing center, raising the possibility that neuronal development may, in addition, require a centrosome‐based actin radial organization. Here, we report, using super‐resolution microscopy and live‐cell imaging of cultured rodent neurons, F‐actin organization around the centrosome with dynamic F‐actin aster‐like structures with F‐actin fibers extending and retracting actively. Photoactivation/photoconversion experiments and molecular manipulations of F‐actin stability reveal a robust flux of somatic F‐actin toward the cell periphery. Finally, we show that somatic F‐actin intermingles with centrosomal PCM‐1 (pericentriolar material 1 protein) satellites. Knockdown of PCM‐1 and disruption of centrosomal activity not only affect F‐actin dynamics near the centrosome but also in distal growth cones. Collectively, the data show a radial F‐actin organization during early neuronal development, which might be a cellular mechanism for providing peripheral regions with a fast and continuous source of actin polymers, hence sustaining initial neuronal development.

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Sprache(n): eng - English
 Datum: 2019-09-062019-01-182019-09-272019-10-242019-12-05
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.15252/embr.201947743
 Art des Abschluß: -

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Projektinformation

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Projektname : We thank Bas van Bommel (M. Mikhaylova's lab, ZMNH, Hamburg), A. Merdes (CNRS, Toulouse), M. Kneussel (ZMNH, Hamburg), M. Harterink (C. Hoogenraad's laboratory, Utrecht), T. Oertner, X. Chai (M. Frotscher's lab, ZMNH, Hamburg), and P. Soba (ZMNH, Hamburg) for antibodies, plasmid constructs, and equipment use. Thanks to I. Hermans-Borgmeyer and members of UKE-Hamburg animal facility, Hamburg for their help with animal experiments and O. Durak (Harvard University) for critical reading of the manuscript. M. Kreutz is supported by the Leibniz Foundation, Deutsche Forschungsgemeinschaft (Kr1879 5-1, 6-1; SFB 779 TPB8), BMBF Energi and JPND STAD. M. Mikhaylova is supported by grants from the Deutsche Forschungsgemeinschaft (DFG Emmy-Noether Programm (Ml 1923/1-1) and FOR2419 (MI 1923/2-1)). O. Kobler is funded by the DFG Grant SCHE 132/18-1. F. Calderon de Anda is supported by Deutsche Forschungsgemeinschaft (DFG) Grants: FOR 2419, CA1495/1-1, and CA 1495/4-1; ERA-NET Neuron Grants (Bundesministerium für Bildung und Forschung, BMBF, 01EW1410, and 01EW1910), JPND Grant (Bundesministerium für Bildung und Forschung, BMBF, 01ED1806), and University Medical Center Hamburg-Eppendorf (UKE). DP. Meka is a co-applicant in the Deutsche Forschungsgemeinschaft (DFG) Grant CA 1495/4-1 for F. Calderon de Anda.
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Quelle 1

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Titel: EMBO Reports
  Andere : EMBO Rep.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Oxford, UK : Published for EMBO by Oxford University Press
Seiten: - Band / Heft: 20 (12) Artikelnummer: e47743 Start- / Endseite: - Identifikator: ISSN: 1469-221X
CoNE: https://pure.mpg.de/cone/journals/resource/110978984569661