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  A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy

Wang, L., Tran, M., D’Este, E., Roberti, J., Koch, B., Xue, L., et al. (2020). A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy. Nature Chemistry, 12, 165-172. doi:10.1038/s41557-019-0371-1.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-4C4D-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-F765-3
Genre: Journal Article

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 Creators:
Wang, Lu1, Author              
Tran, Mai1, Author              
D’Este, Elisa, Author
Roberti, Julia, Author
Koch, Birgit1, Author              
Xue, Lin, Author
Johnsson, Kai1, Author              
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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Free keywords: Chemical tools ; Fluorescent dyes ; Imaging studies ; Super-resolution microscopy
 Abstract: Live-cell fluorescence nanoscopy is a powerful tool to study cellular biology on a molecular scale, yet its use is held back by the paucity of suitable fluorescent probes. Fluorescent probes based on regular fluorophores usually suffer from a low cell permeability and an unspecific background signal. Here we report a general strategy to transform regular fluorophores into fluorogenic probes with an excellent cell permeability and a low unspecific background signal. Conversion of a carboxyl group found in rhodamines and related fluorophores into an electron-deficient amide does not affect the spectroscopic properties of the fluorophore, but allows us to rationally tune the dynamic equilibrium between two different forms: a fluorescent zwitterion and a non-fluorescent, cell-permeable spirolactam. Furthermore, the equilibrium generally shifts towards the fluorescent form when the probe binds to its cellular targets. The resulting increase in fluorescence can be up to 1,000-fold. Using this simple design principle, we created fluorogenic probes in various colours for different cellular targets for wash-free, multicolour, live-cell nanoscopy.

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Language(s): eng - English
 Dates: 2019-05-252019-10-092019-12-022020-02-01
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41557-019-0371-1
 Degree: -

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Title: Nature Chemistry
  Abbreviation : Nat. Chem.
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 12 Sequence Number: - Start / End Page: 165 - 172 Identifier: ISSN: 1755-4330
CoNE: https://pure.mpg.de/cone/journals/resource/1755-4330