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  Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system.

Gregor, C., Pape, J. K., Gwosch, K., Gilat, T., Sahl, S. J., & Hell, S. W. (2019). Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system. Proceedings of the National Academy of Sciences of the United States of America, 116(52), 26491-26496. doi:10.1073/pnas.1913616116.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-4F0F-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-795F-A
Genre: Journal Article

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 Creators:
Gregor, C.1, Author              
Pape, J. K.1, Author              
Gwosch, K.1, Author              
Gilat, T.1, Author              
Sahl, S. J.1, Author              
Hell, S. W.1, Author              
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1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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Free keywords: bioluminescence; luciferase; lux; microscopy
 Abstract: Bioluminescence-based imaging of living cells has become an important tool in biological and medical research. However, many bioluminescence imaging applications are limited by the requirement of an externally provided luciferin substrate and the low bioluminescence signal which restricts the sensitivity and spatiotemporal resolution. The bacterial bioluminescence system is fully genetically encodable and hence produces autonomous bioluminescence without an external luciferin, but its brightness in cell types other than bacteria has, so far, not been sufficient for imaging single cells. We coexpressed codon-optimized forms of the bacterial luxCDABE and frp genes from multiple plasmids in different mammalian cell lines. Our approach produces high luminescence levels that are comparable to firefly luciferase, thus enabling autonomous bioluminescence microscopy of mammalian cells.

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Language(s): eng - English
 Dates: 2019-12-022019-12-26
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1913616116
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Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Pages: - Volume / Issue: 116 (52) Sequence Number: - Start / End Page: 26491 - 26496 Identifier: -