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  On the use of different nucleic acid precursors for the measurement of microbial nucleic acid turnover

Witzel, K.-P., & Graf, G. (1984). On the use of different nucleic acid precursors for the measurement of microbial nucleic acid turnover. Ergebnisse der Limnologie/Advances in Limnology, 19, 59-65.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-5376-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-5377-8
Genre: Journal Article

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Witzel, Karl-Paul1, Author              
Graf, G.2, Author
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1Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_976547              
2External Organizations, ou_persistent22              

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 Abstract: The incorporation of the following nucleic acid precursors into nucleic acids of the microbial community from lake Großer Plöner See was measured, (methyl-3 H)thymidine, (5,6-3 H)uridine, (methyl-3H)thymine, (2-3H)adenine, (5-3H)orotic acid, L-(U-14C)aspartic acid, (U-14C)glycine, D-(l-14C)ribose and 32P phosphate. RNA and DNA were enriched and purified by adsorption onto hydroxyapatite and subsequent elution with phosphate buffers of increasing molarity. All of the precursors tested were incorporated into RNA and DNA. There was a significant labeling of other cell substances, too. The rates of RNA ¿nd DNA synthesis calculated from radioactive uptake showed large variations between different precursors.

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Language(s): eng - English
 Dates: 1984-12
 Publication Status: Published in print
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Title: Ergebnisse der Limnologie/Advances in Limnology
  Other : Arch. Hydrobiol. Beih. Ergebn. Limnol.
Source Genre: Journal
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Publ. Info: Stuttgart : E. Schweizerbart'sche Verlagsbuchhandlung
Pages: - Volume / Issue: 19 Sequence Number: - Start / End Page: 59 - 65 Identifier: ISSN: 0071-1128
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000017140