English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Can mixed cultures help the understanding of natural heterotrophic processes?

Höfle, M. G. (1984). Can mixed cultures help the understanding of natural heterotrophic processes? Ergebnisse der Limnologie/Advances in Limnology, 19, 53-58.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-538C-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-538D-F
Genre: Journal Article

Files

show Files
hide Files
:
Höfle_1984.pdf (Publisher version), 4MB
 
File Permalink:
-
Name:
Höfle_1984.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Evolutionary Biology, MPLM; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Höfle, Manfred G.1, Author              
Affiliations:
1Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_976547              

Content

show
hide
Free keywords: -
 Abstract: Batch cultures of natural bacterial communities were grown in filter-sterilized seawater from coastal and open-ocean sites. Natural coastal seawater was supplemented with up to 500 μg/l of various nitrogen containing substrates like putrescine, cadaverine, glutamic acid, serine and ammonia. The removal of these compounds was monitored by high performance liquid chromatography. Increases in bacterial cell number during the batch culture were counted with an epifluorescence microscope after acridine organe staining (AODC). All compounds were removed from coastal seawater cultures within 48 hours with no corresponding increase in bacterial yield or growth rate relative to control (unsupplemented) cultures. Shipboard experiments with open-ocean deep water (1500 m) showed similar results, but slower removal of the added substrates from seawater. Indicators of metabolic activity such as glucose and glutamic acid uptake and mineralization potentials per cell and ratio of colony forming units (CFU) to total cell counts (AODC) increased markedly during the growth phase. This mixed culture approach could therefore help estimate heterotrophic potentials of compounds that are difficult to degrade over reasonable incubation times with natural bacterial communities and also elucidate the biochemical fate of these compounds.

Details

show
hide
Language(s): eng - English
 Dates: 1984-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Ergebnisse der Limnologie/Advances in Limnology
  Other : Arch. Hydrobiol. Beih. Ergebn. Limnol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Stuttgart : E. Schweizerbart'sche Verlagsbuchhandlung
Pages: - Volume / Issue: 19 Sequence Number: - Start / End Page: 53 - 58 Identifier: ISSN: 0071-1128
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000017140