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  Germline-Transmitted Genome Editing Methodology in Arabidopsis thaliana Using TAL Effector Nucleases

Forner, J. (2020). Germline-Transmitted Genome Editing Methodology in Arabidopsis thaliana Using TAL Effector Nucleases. In M. Naseem, & T. Dandekar (Eds.), Plant Stem Cells: Methods and Protocols (pp. 23-30). New York, NY: Springer US.

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 Creators:
Forner, J.1, Author           
Affiliations:
1Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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 Abstract: TAL effector nucleases (TALENs) are powerful tools to create specific knockout mutants in plants. The use of an optimized TALEN backbone and the choice of promoters that are strongly active in the stem cells of the shoot apical meristem are key to a high rate of heritable targeted mutations. Recommendations for construct design and screening for mutants are given in this chapter.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-0716-0183-9_3
BibTex Citekey: Forner2020
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Title: Plant Stem Cells: Methods and Protocols
Source Genre: Book
 Creator(s):
Naseem, Muhammad, Editor
Dandekar, Thomas, Editor
Affiliations:
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Publ. Info: New York, NY : Springer US
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 23 - 30 Identifier: ISBN: 978-1-0716-0183-9