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  Identification of regulatory networks in HSCs and their immediate progeny via integrated proteome, transcriptome, and DNA methylome analysis

Cabezas-Wallscheid, N., Klimmeck, D., Hansson, J., Lipka, D. B., Reyes, A., Wang, Q., et al. (2014). Identification of regulatory networks in HSCs and their immediate progeny via integrated proteome, transcriptome, and DNA methylome analysis. Cell Stem Cell, 15, 507-522. doi:org/10.1016/j.stem.2014.07.005.

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https://pdf.sciencedirectassets.com/274143/1-s2.0-S1934590914X0010X/1-s2.0-S1934590914003014/main.pdf?X-Amz-Date=20191210T094225Z&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Signature=b6ce98f7a7d040c7f765b0c3f7bb7b325ce3f2c2eadedf0e681303d76aadcbf0&X-Amz-Credential=ASIAQ3PHCVTY752BZ5OW%2F20191210%2Fus-east-1%2Fs3%2Faws4_request&type=client&tid=prr-1977a852-d08e-4504-9d24-c6a14258c60c&sid=eb2c4b8973f4304cb73811d8e548349985c5gxrqb&pii=S1934590914003014&X-Amz-SignedHeaders=host&X-Amz-Security-Token=IQoJb3JpZ2luX2VjEIn%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJGMEQCIGkBGTy0Vja93JVmdFFWUdtjwCEVKpjDQV5UV6JI0v9kAiApY41zuAwdJ2RkK5YNNEEU7tnJMx6fCOcOKkp9x%2BQ2gSrYAgjS%2F%2F%2F%2F%2F%2F%2F%2F%2F%2F8BEAIaDDA1OTAwMzU0Njg2NSIMe3S2D24lJD1ix9zMKqwCA1gKVnUX0rdZC2VVY4grQd160BEtBXMFa5HTaIW%2Fo4srt919lRkY%2BH93tzx4MNB0sjIh%2Bn1RRn%2F4je8fvCDXIFRPr4zQXrDEV4nYZDmc6Mp9XOt%2BloMsVfEZWXxN3ytn8DegKJ%2Bs6v65XDtZxCEI86pEgEGqQM5Vl1Z%2FFFD7ueoR0Dzs2aSt4m6us4xV5vDDCtYRFmJkm4OOMnekWjpS6MLOYIm%2BR910VbCEtUJeJFJ8XyTo9GkFSNp2BMsbhEDe%2BvdU0zvoa7yVwovJUTgo%2Fi5vF3UBZdqcVGe8xQVQ584AyLhoEdrM64PeR6dR9N8zLq8y4bedXVwYe7UyITG2pvBDFb4BUDwMOpnK01kgINfhVIaw%2BcA4Xw1mjZtogOqgqaooAK9Vg6DrPg35MOa7ve8FOtECmyfzoR5mpOfg93VsQRPBUXZqsvg4pAjpKpc5H2Bvlmy4RMPF7sz406bEbHPpGIhZ7C9xQvmwuvmg4eKqBA7UwHqt3ttdITfNfuQgSM8bO5fCen920fQc1TRL5iQUOyvzb70%2FM52lo6NzM2k0wAQG4UyT5qhMySrg9fnv132e8x1FhQVbCUzAubTgqSCIrGEYTleknn7voeFkdhWK8%2FcxLdvTkeHUeUbUkUz%2Bo3OUcMOWMcLsCcMGvQYjHhRBGQTt0yjAg60kB5KaBn8ysiX3NGvXAdv0vlx52I3X5QlXN2fmfFSjtPNu0GwXin%2B2BQtwJJ%2FSSw0dbaav2SMJ0WXf0s4aHj8nTF7FHS%2BpUcGHK2%2BW8cw0Ko99d5mF8gtgyX8cGHo7%2BfX%2FLutaeJzh0Y15Yg2FlPdxDWWcFHOwfSW7KeuPnGazglWVvjtjZWwR245Ukw%3D%3D&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&X-Amz-Expires=300&hash=4818f8f009656e75ff03df8022bce63a4ec041bbc27a0c6ee9076894bb287338 (Publisher version)
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Cabezas-Wallscheid, Nina1, Author           
Klimmeck, Daniel2, Author
Hansson, Jenny2, Author
Lipka, Daniel B.2, Author
Reyes, Alejandro2, Author
Wang, Qi2, Author
Weichenhan, Dieter2, Author
Lier, Amelie2, Author
von Paleske, Lisa2, Author
Renders, Simon2, Author
Wünsche, Peer2, Author
Zeisberger, Petra2, Author
Brocks, David2, Author
Gu, Lei2, Author
Herrmann, Carl2, Author
Haas, Simon2, Author
Essers, Marieke A.G.2, Author
Brors, Benedikt2, Author
Trumpp, Andreas2, Author
Affiliations:
1Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, ou_2243641              
2External Organizations, ou_persistent22              

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 Abstract: In this study, we present integrated quantitative proteome, transcriptome, and methylome analyses of hematopoietic stem cells (HSCs) and four multipotent progenitor (MPP) populations. From the characterization of more than 6,000 proteins, 27,000 transcripts, and 15,000 differentially methylated regions (DMRs), we identified coordinated changes associated with early differentiation steps. DMRs show continuous gain or loss of methylation during differentiation, and the overall change in DNA methylation correlates inversely with gene expression at key loci. Our data reveal the differential expression landscape of 493 transcription factors and 682 lncRNAs and highlight specific expression clusters operating in HSCs. We also found an unexpectedly dynamic pattern of transcript isoform regulation, suggesting a critical regulatory role during HSC differentiation, and a cell cycle/DNA repair signature associated with multipotency in MPP2 cells. This study provides a comprehensive genome-wide resource for the functional exploration of molecular, cellular, and epigenetic regulation at the top of the hematopoietic hierarchy.

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Language(s): eng - English
 Dates: 2014
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: org/10.1016/j.stem.2014.07.005
 Degree: -

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Title: Cell Stem Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 15 Sequence Number: - Start / End Page: 507 - 522 Identifier: ISSN: 1934-5909
CoNE: https://pure.mpg.de/cone/journals/resource/1934-5909