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  A target-driven DNA-based molecular machine for rapid and homogeneous detection of arginine-vasopressin

Tan, H., Chen, L., Li, X., Li, M., & Zhao, M. (2020). A target-driven DNA-based molecular machine for rapid and homogeneous detection of arginine-vasopressin. Analyst, 145(3), 880-886. doi:10.1039/C9AN02060H.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-61B0-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-A8EE-2
Genre: Journal Article

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 Creators:
Tan, Haocheng, Author
Chen, Lu1, Author              
Li, Xinyi, Author
Li, Mengyuan, Author
Zhao, Meiping, Author
Affiliations:
1Markus Antonietti, Kolloidchemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863321              

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 Abstract: Rapid detection of physiological changes of neuropeptides is of great importance as they are involved in a wide range of physiological processes and behaviors. Abnormalities in their expression level are correlated with various neurological diseases. However, current methods such as radioimmunoassay, enzyme-linked immunosorbent assays and liquid chromatography tandem mass spectrometry relied on cumbersome operation steps and could not rapidly provide the information of their concentration fluctuations. Thus motivated, we developed a target-driven DNA-based molecular machine that could be triggered only in the presence of a specific target neuropeptide. Using arginine-vasopressin (AVP) as a model neuropeptide, we integrated the DNA-based molecular machine with fluorescence signal transduction and amplification technology. The assay was rapid and homogeneous, which offered a linear range of 75–700 pM and a limit-of-detection as low as 75 pM. It holds great potential for further applications in real-time monitoring of the variations of the AVP level in biological samples.

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Language(s): eng - English
 Dates: 2019-11-212020
 Publication Status: Published in print
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 Identifiers: DOI: 10.1039/C9AN02060H
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Title: Analyst
  Abbreviation : Analyst
Source Genre: Journal
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Publ. Info: London : Royal Society of Chemistry
Pages: - Volume / Issue: 145 (3) Sequence Number: - Start / End Page: 880 - 886 Identifier: ISSN: 0003-2654