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  Tricalbin-Mediated Contact Sites Control ER Curvature to Maintain Plasma Membrane Integrity

Collado, J., Kalemanov, M., Campelo, F., Bourgoint, C., Thomas, F., Loewith, R., et al. (2019). Tricalbin-Mediated Contact Sites Control ER Curvature to Maintain Plasma Membrane Integrity. DEVELOPMENTAL CELL, 51(4), 476-487.e7. doi:10.1016/j.devcel.2019.10.018.

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 Creators:
Collado, Javier1, Author              
Kalemanov, Maria1, Author              
Campelo, Felix2, Author
Bourgoint, Clelia2, Author
Thomas, Ffion2, Author
Loewith, Robbie2, Author
Martinez Sanchez, Antonio1, Author              
Baumeister, Wolfgang1, Author              
Stefan, Christopher J.2, Author
Fernandez-Busnadiego, Ruben1, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
2external, ou_persistent22              

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Free keywords: CORTICAL ENDOPLASMIC-RETICULUM; CRYOELECTRON TOMOGRAPHY; SACCHAROMYCES-CEREVISIAE; EXTENDED SYNAPTOTAGMINS; PROTEINS; YEAST; ARABIDOPSIS; BINDING; MECHANISMS; LIPIDS
 Abstract: Membrane contact sites (MCS) between the endoplasmic reticulum (ER) and the plasma membrane (PM) play fundamental roles in all eukaryotic cells. ER-PM MCS are particularly abundant in Saccharomyces cerevisiae, where approximately half of the PM surface is covered by cortical ER (cER). Several proteins, including Ist2, Scs2/22, and Tcb1/2/3 are implicated in cER formation, but the specific roles of these molecules are poorly understood. Here, we use cryo-electron tomography to show that ER-PM tethers are key determinants of cER morphology. Notably, Tcb proteins (tricalbins) form peaks of extreme curvature on the cER membrane facing the PM. Combined modeling and functional assays suggest that Tcb-mediated cER peaks facilitate the transport of lipids between the cER and the PM, which is necessary to maintain PM integrity under heat stress. ER peaks were also present at other MCS, implying that membrane curvature enforcement may be a widespread mechanism to regulate MCS function.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 19
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: DEVELOPMENTAL CELL
Source Genre: Journal
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Publ. Info: 50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 51 (4) Sequence Number: - Start / End Page: 476 - 487.e7 Identifier: ISSN: 1534-5807