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  Actin Waves and Dynamic Patterning of the Plasma Membrane

Gerisch, G., Prassler, J., Butterfield, N., & Ecke, M. (2019). Actin Waves and Dynamic Patterning of the Plasma Membrane. YALE JOURNAL OF BIOLOGY AND MEDICINE, 92(3), 397-411.

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 Creators:
Gerisch, Günther1, Author           
Prassler, Jana1, Author           
Butterfield, Nelson2, Author           
Ecke, Mary1, Author           
Affiliations:
1Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565149              
2Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: DICTYOSTELIUM MUTANTS LACKING; CYTOSKELETON DYNAMICS; FLUORESCENT PROTEIN; PIP3 WAVES; CELLS; CORONIN; RAS; CHEMOATTRACTANT; ACTIVATION; RELOCALIZATION
 Abstract: Plasma membrane and underlying actin network are connected to a functional unit that by non-linear interactions is capable of forming patterns. For instance, in cell motility and chemotaxis, cells polarize to form a protruding front and a retracting tail. Here we address dynamic patterns that are formed on a planar substrate surface and are therefore easily accessible to optical recording. In these patterns two distinct areas of the membrane and actin cortex are interconverted at the site of circular actin waves. The inner territory circumscribed by a wave is distinguished from the external area by a high PIP3 content and high Ras activity. In contrast, the external area is occupied with the PIP3-degrading phosphatase PTEN. In the underlying cortex, these areas differ in the proteins associated with the actin network. Actin waves can be formed at zones of increasing as well as decreasing Ras activity. Both types of waves are headed by myosin IB. When waves collide, they usually extinguish each other, and their decay is accompanied by the accumulation of coronin. No membrane patterns have been observed after efficient depolymerization of actin, suggesting that residual actin filaments are necessary for the pattern generating system to work. Where appropriate, we relate the experimental data obtained with Dictyostelium to human normal and malignant cell behavior, in particular to the role of Ras-GAP as an enhancer of macropinocytosis, to mutations in the tumor suppressor PTEN, to frustrated phagocytosis, and to the role of coronin in immune cells and neurons.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000487261800004
 Degree: -

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Title: YALE JOURNAL OF BIOLOGY AND MEDICINE
Source Genre: Journal
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Publ. Info: 333 CEDAR ST, PO BOX 208000, NEW HAVEN, CT 06520-8000 USA : YALE J BIOLOGY MEDICINE, INC
Pages: - Volume / Issue: 92 (3) Sequence Number: - Start / End Page: 397 - 411 Identifier: ISSN: 0044-0086