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  Ablation of CNTN2+Pyramidal Neurons During Development Results in Defects in Neocortical Size and Axonal Tract Formation

Kastriti, M. E., Stratigi, A., Mariatos, D., Theodosiou, M., Savvaki, M., Kavkova, M., et al. (2019). Ablation of CNTN2+Pyramidal Neurons During Development Results in Defects in Neocortical Size and Axonal Tract Formation. FRONTIERS IN CELLULAR NEUROSCIENCE, 13: 454. doi:10.3389/fncel.2019.00454.

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 Creators:
Kastriti, Maria Eleni1, Author
Stratigi, Aikaterini1, Author
Mariatos, Dimitris1, Author
Theodosiou, Marina2, Author              
Savvaki, Maria1, Author
Kavkova, Michaela1, Author
Theodorakis, Kostas1, Author
Vidaki, Marina1, Author
Zikmund, Tomas1, Author
Kaiser, Jozef1, Author
Adameyko, Igor1, Author
Karagogeos, Domna1, Author
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1external, ou_persistent22              
2Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              

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Free keywords: ADHESION MOLECULE TAG-1; THALAMOCORTICAL AXONS; GLYCOPROTEIN TAG-1; PROJECTION NEURONS; EXPRESSION; MIGRATION; TBR1; OLIGODENDROCYTES; GROWTH; CELLSCNTN2; TAG-1; corticofugal system; corticothalamic axons; anterior commissure; corpus callosum; central nervous system;
 Abstract: Corticothalamic axons express Contactin-2 (CNTN2/TAG-1), a neuronal recognition molecule of the immunoglobulin superfamily involved in neurogenesis, neurite outgrowth, and fasciculation. TAG-1, which is expressed transiently by cortical pyramidal neurons during embryonic development, has been shown to be fundamental for axonal recognition, cellular migration, and neuronal proliferation in the developing cortex. Although Tag-1(-/-) mice do not exhibit any obvious defects in the corticofugal system, the role of TAG-1+ neurons during the development of the cortex remains elusive. We have generated a mouse model expressing EGFP under the Tag-1 promoter and encompassing the coding sequence of Diptheria Toxin subunit A (DTA) under quiescence with no effect on the expression of endogenous Tag-1. We show that while the line recapitulates the expression pattern of the molecule, it highlights an extended expression in the forebrain, including multiple axonal tracts and neuronal populations, both spatially and temporally. Crossing these mice to the Emx1-Cre strain, we ablated the vast majority of TAG-1+ cortical neurons. Among the observed defects were a significantly smaller cortex, a reduction of corticothalamic axons as well as callosal and commissural defects. Such defects are common in neurodevelopmental disorders, thus this mouse could serve as a useful model to study physiological and pathophysiological cortical development.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published online
 Pages: 19
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000497726700001
DOI: 10.3389/fncel.2019.00454
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Title: FRONTIERS IN CELLULAR NEUROSCIENCE
Source Genre: Journal
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Publ. Info: AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND : FRONTIERS MEDIA SA
Pages: - Volume / Issue: 13 Sequence Number: 454 Start / End Page: - Identifier: -