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  Structural basis of poxvirus transcription: Vaccinia RNA polymerase complexes.

Grimm, C., Hillen, H. S., Bedenk, K., Bartuli, J., Neyer, S., Zhang, Q., et al. (2019). Structural basis of poxvirus transcription: Vaccinia RNA polymerase complexes. Cell, 179(7), 1537-1550. doi:10.1016/j.cell.2019.11.024.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-5D8D-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-5D8F-3
Genre: Journal Article

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 Creators:
Grimm, C., Author
Hillen, H. S.1, Author              
Bedenk, K., Author
Bartuli, J., Author
Neyer, S.1, Author              
Zhang, Q., Author
Hüttenhofer, A., Author
Erlacher, M., Author
Dienemann, C.1, Author              
Schlosser, A., Author
Urlaub, H.2, Author              
Böttcher, B., Author
Szalay, A. A., Author
Cramer, P.1, Author              
Fischer, U., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              
2Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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Free keywords: DNA-dependent RNA polymerase; RNA polymerase; cryo electron microscopy; cryo-EM; cryo-EM reconstruction; poxviridae; single particle; structure; transcription; vaccinia
 Abstract: Poxviruses encode a multisubunit DNA-dependent RNA polymerase (vRNAP) that carries out viral gene expression in the host cytoplasm. We report cryo-EM structures of core and complete vRNAP enzymes from Vaccinia virus at 2.8 Å resolution. The vRNAP core enzyme resembles eukaryotic RNA polymerase II (Pol II) but also reveals many virus-specific features, including the transcription factor Rap94. The complete enzyme additionally contains the transcription factor VETF, the mRNA processing factors VTF/CE and NPH-I, the viral core protein E11, and host tRNAGln. This complex can carry out the entire early transcription cycle. The structures show that Rap94 partially resembles the Pol II initiation factor TFIIB, that the vRNAP subunit Rpo30 resembles the Pol II elongation factor TFIIS, and that NPH-I resembles chromatin remodeling enzymes. Together with the accompanying paper (Hillen et al., 2019), these results provide the basis for unraveling the mechanisms of poxvirus transcription and RNA processing.

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Language(s): eng - English
 Dates: 2019-12-12
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cell.2019.11.024
 Degree: -

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Title: Cell
Source Genre: Journal
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Pages: - Volume / Issue: 179 (7) Sequence Number: - Start / End Page: 1537 - 1550 Identifier: -