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  Structural basis of poxvirus transcription: Transcribing and capping vaccinia complexes.

Hillen, H. S., Bartuli, J., Grimm, C., Dienemann, C., Bedenk, K., Szalay, A. A., et al. (2019). Structural basis of poxvirus transcription: Transcribing and capping vaccinia complexes. Cell, 179(7), 1525-1536. doi:10.1016/j.cell.2019.11.023.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-5DA8-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-5DAA-4
Genre: Journal Article

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 Creators:
Hillen, H. S.1, Author              
Bartuli, J., Author
Grimm, C., Author
Dienemann, C.1, Author              
Bedenk, K., Author
Szalay, A. A., Author
Fischer, U., Author
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: DNA-dependent RNA polymerase; Poxviridae; RNA polymerase; Vaccinia; capping enzyme; cryo electron microscopy; cryo-EM; elongation; structure; transcription
 Abstract: Poxviruses use virus-encoded multisubunit RNA polymerases (vRNAPs) and RNA-processing factors to generate m7G-capped mRNAs in the host cytoplasm. In the accompanying paper, we report structures of core and complete vRNAP complexes of the prototypic Vaccinia poxvirus (Grimm et al., 2019; in this issue of Cell). Here, we present the cryo-electron microscopy (cryo-EM) structures of Vaccinia vRNAP in the form of a transcribing elongation complex and in the form of a co-transcriptional capping complex that contains the viral capping enzyme (CE). The trifunctional CE forms two mobile modules that bind the polymerase surface around the RNA exit tunnel. RNA extends from the vRNAP active site through this tunnel and into the active site of the CE triphosphatase. Structural comparisons suggest that growing RNA triggers large-scale rearrangements on the surface of the transcription machinery during the transition from transcription initiation to RNA capping and elongation. Our structures unravel the basis for synthesis and co-transcriptional modification of poxvirus RNA.

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Language(s): eng - English
 Dates: 2019-12-12
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cell.2019.11.023
 Degree: -

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Title: Cell
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 179 (7) Sequence Number: - Start / End Page: 1525 - 1536 Identifier: -