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  A biosensor for measuring NAD+ levels at the point of care

Yu, Q., Pourmandi, N., Xue, L., Gondrand, C., Fabritz, S., Bardy, D., et al. (2019). A biosensor for measuring NAD+ levels at the point of care. nature metabolism, 1219-1225. doi:10.1038/s42255-019-0151-7.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0005-6119-2 Version Permalink: https://hdl.handle.net/21.11116/0000-0005-611A-1
Genre: Journal Article

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 Creators:
Yu, Qiuliyang1, Author           
Pourmandi, Narges2, Author           
Xue, Lin1, Author           
Gondrand, Corentin2, Author           
Fabritz, Sebastian1, Author           
Bardy, Daniel, Author
Patiny, Luc, Author
Katsyuba, Elena, Author
Auwerx, Johan, Author
Johnsson, Kai1, Author           
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              
2Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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 Abstract: The cellular level of nicotinamide adenine dinucleotide (NAD+), through its different functions, affects cellular metabolism and signalling1,2,3. A decrease in the NAD+ content has been associated with various pathologies and physiological aging4,5, while strategies to boost cellular NAD+ levels have been shown to be effective against age-related diseases in many animal models6. The link between decreased NAD+ levels and numerous pathologies and physiological aging has triggered the need for a simple quantification method for NAD+, ideally applicable at the point of care. Here, we introduce a bioluminescent biosensor for the rapid quantification of NAD+ levels in biological samples, which can be used either in laboratories or at the point of care. The biosensor is a semisynthetic, light-emitting sensor protein that changes the colour of emitted light from blue to red on binding of NAD+. This NAD+-dependent colour change enables the use of the biosensor in paper-based assays in which NAD+ is quantified by measuring the colour of the emitted light by using either a simple digital camera or a plate reader. We used the approach to quantify NAD+ levels in cell culture, tissue and blood samples, yielding results that agreed with those from standard testing methods. The same biosensor furthermore allows the quantification of NAD+-dependent enzymatic activities in blood samples, thus expanding its utility as a tool for point-of-care diagnostics.

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Language(s): eng - English
 Dates: 2019-04-182019-11-122019-12-132019-12-13
 Publication Status: Issued
 Pages: 19
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s42255-019-0151-7
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Title: nature metabolism
Source Genre: Journal
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Publ. Info: London : Springer Nature
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 1219 - 1225 Identifier: ISSN: 2522-5812