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  Analyzing the substrate specificity of a novel class of, longhorned beetle-derived, xylanases using synthetic arabinoxylan oligo- and polysaccharides

Pauchet, Y., Ruprecht, C., & Pfrengle, F. (2020). Analyzing the substrate specificity of a novel class of, longhorned beetle-derived, xylanases using synthetic arabinoxylan oligo- and polysaccharides. Chembiochem, 21, 1517-1525. doi:10.1002/cbic.201900687.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-6D58-F Version Permalink: http://hdl.handle.net/21.11116/0000-0006-8705-C
Genre: Journal Article

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 Creators:
Pauchet, Yannick, Author
Ruprecht, Colin1, Author              
Pfrengle, Fabian1, Author              
Affiliations:
1Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863303              

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Free keywords: Enzyme, xylanase, GH5_2, insect, synthetic oligosaccharides
 Abstract: Xylophagous longhorned beetles thrive in challenging environments. To access nutrients, they secrete plant-cell-wall-degrading enzymes in their gut fluid, among them, cellulases of the subfamily 2 of glycoside hydrolase family 5 (GH5\_2). Recently, we discovered that several beetle-derived GH5\_2s use xylan as a substrate instead of cellulose, which is unusual for this family of enzymes. Here, we analyze the substrate specificity of a GH5\_2 xylanase from the beetle Apriona japonica (AJAGH5\_2-1) using commercially available substrates and synthetic arabinoxylan oligo- and polysaccharides. We demonstrate that AJAGH5\_2-1 deals with arabinoxylan polysaccharides in a way that is distinct from classical xylanase families like GH10 and GH11. AJAGH5\_2-1 is active on long oligosaccharides and cleaves at the non-reducing end of a substituted xylose residue (position +1) only if (i) three xylose residues are present upstream and downstream of the cleavage site and (ii) xylose residues at positions -1, -2, +2 and +3 are not substituted.

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Language(s): eng - English
 Dates: 2019-12-182020
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1002/cbic.201900687
BibTex Citekey: doi:10.1002/cbic.201900687
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Title: Chembiochem
  Other : Chembiochem
Source Genre: Journal
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Publ. Info: Weinheim, Germany : Wiley-VCH
Pages: - Volume / Issue: 21 Sequence Number: - Start / End Page: 1517 - 1525 Identifier: ISSN: 1439-4227