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  Distinct Roles for Condensin's Two ATPase Sites in Chromosome Condensation

Elbatsh, A. M. O., Kim, E., Eeftens, J. M., Raaijmakers, J. A., van der Weide, R. H., Garcia-Nieto, A., et al. (2019). Distinct Roles for Condensin's Two ATPase Sites in Chromosome Condensation. MOLECULAR CELL, 76(5), 724-737.e5. doi:10.1016/j.molcel.2019.09.020.

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 Creators:
Elbatsh, Ahmed M. O.1, Author
Kim, Eugene1, Author
Eeftens, Jorine M.1, Author
Raaijmakers, Jonne A.1, Author
van der Weide, Robin H.1, Author
Garcia-Nieto, Alberto1, Author
Bravo, Sol1, Author
Ganji, Mahipal2, Author              
de Bos, Jelmi Uit1, Author
Teunissen, Hans1, Author
Medema, Rene H.1, Author
de Wit, Elzo1, Author
Haering, Christian H.1, Author
Dekker, Cees1, Author
Rowland, Benjamin D.1, Author
Affiliations:
1external, ou_persistent22              
2Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              

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Free keywords: SISTER-CHROMATID RESOLUTION; REAL-TIME DETECTION; STRUCTURAL BASIS; DNA COMPACTION; SMC; BINDING; PROTEIN; ORGANIZATION; ASSOCIATION; COMPLEX
 Abstract: Condensin is a conserved SMC complex that uses its ATPase machinery to structure genomes, but how it does so is largely unknown. We show that condensin's ATPase has a dual role in chromosome condensation. Mutation of one ATPase site impairs condensation, while mutating the second site results in hyperactive condensin that compacts DNA faster than wild-type, both in vivo and in vitro. Whereas one site drives loop formation, the second site is involved in the formation of more stable higher-order Z loop structures. Using hyperactive condensin I, we reveal that condensin II is not intrinsically needed for the shortening of mitotic chromosomes. Condensin II rather is required for a straight chromosomal axis and enables faithful chromosome segregation by counteracting the formation of ultrafine DNA bridges. SMC complexes with distinct roles for each ATPase site likely reflect a universal principle that enables these molecular machines to intricately control chromosome architecture.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 19
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: MOLECULAR CELL
Source Genre: Journal
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Publ. Info: 50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 76 (5) Sequence Number: - Start / End Page: 724 - 737.e5 Identifier: ISSN: 1097-2765