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  SILAC-based quantitative proteomics using mass spectrometry quantifies endoplasmic reticulum stress in whole HeLa cells

Itzhak, D. N., Sacco, F., Nagaraj, N., Tyanova, S., Mann, M., & Murgia, M. (2019). SILAC-based quantitative proteomics using mass spectrometry quantifies endoplasmic reticulum stress in whole HeLa cells. DISEASE MODELS & MECHANISMS, 12(11): UNSP dmm040741. doi:10.1242/dmm.040741.

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 Creators:
Itzhak, Daniel N.1, Author              
Sacco, Francesca1, Author              
Nagaraj, Nagarjuna1, Author              
Tyanova, Stefka1, Author              
Mann, Matthias1, Author              
Murgia, Marta1, Author              
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1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: UNFOLDED PROTEIN RESPONSE; ER-STRESS; MESSENGER-RNA; GENE-EXPRESSION; XBP1; ATF6; SEQUENCE; REVEALS; DECAY; IRE1Proteomics; SILAC; Unfolded protein response; Endoplasmic reticulum stress; Tunicamycin;
 Abstract: The unfolded protein response (UPR) involves extensive proteome remodeling in many cellular compartments. To date, a comprehensive analysis of the UPR has not been possible because of technological limitations. Here, we employ stable isotope labeling with amino acids in cell culture (SILAC)-based proteomics to quantify the response of over 6200 proteins to increasing concentrations of tunicamycin in HeLa cells. We further compare the effects of tunicamycin (5 mu g/ml) to those of thapsigargin (1 mu M) and DTT (2 mM), both activating the UPR through different mechanisms. This systematic quantification of the proteome-wide expression changes that follow proteostatic stress is a resource for the scientific community, enabling the discovery of novel players involved in the pathophysiology of the broad range of disorders linked to proteostasis. We identified increased expression in 38 proteins not previously linked to the UPR, of which 15 likely remediate ER stress, and the remainder may contribute to pathological outcomes. Unexpectedly, there are few strongly downregulated proteins, despite expression of the pro-apoptotic transcription factor CHOP, suggesting that IRE1-dependent mRNA decay (RIDD) has a limited contribution to ER stress-mediated cell death in our system.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published online
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000500692600006
DOI: 10.1242/dmm.040741
 Degree: -

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Title: DISEASE MODELS & MECHANISMS
Source Genre: Journal
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Publ. Info: BIDDER BUILDING, STATION RD, HISTON, CAMBRIDGE CB24 9LF, ENGLAND : COMPANY BIOLOGISTS LTD
Pages: - Volume / Issue: 12 (11) Sequence Number: UNSP dmm040741 Start / End Page: - Identifier: ISSN: 1754-8403