English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Micronuclei-based model system reveals functional consequences of chromothripsis in human cells

Kneissig, M., Keuper, K., de Pagter, M. S., van Roosmalen, M. J., Martin, J., Otto, H., et al. (2019). Micronuclei-based model system reveals functional consequences of chromothripsis in human cells. ELIFE, 8: e50292. doi:10.7554/eLife.50292.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
elife-50292-v2.pdf (Any fulltext), 3MB
Name:
elife-50292-v2.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
open access article
License:
-

Locators

show

Creators

show
hide
 Creators:
Kneissig, Maja1, Author
Keuper, Kristina1, Author
de Pagter, Mirjam S.1, Author
van Roosmalen, Markus J.1, Author
Martin, Jana1, Author
Otto, Hannah1, Author
Passerini, Verena2, Author              
Sparr, Aline Campos2, Author              
Renkens, Ivo1, Author
Kropveld, Fenna1, Author
Vasudevan, Anand1, Author
Sheltzer, Jason M.1, Author
Kloosterman, Wigard P.1, Author
Storchova, Zuzana2, Author              
Affiliations:
1external, ou_persistent22              
2Storchova, Zuzana / Maintenance of Genome Stability, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565171              

Content

show
hide
Free keywords: NUCLEAR-ENVELOPE RUPTURE; DNA-DAMAGE; CANCER; REARRANGEMENTS; REPAIR; CHROMOSOMES; MECHANISMS; GERMLINE; ERRORS; BREAKS
 Abstract: Cancer cells often harbor chromosomes in abnormal numbers and with aberrant structure. The consequences of these chromosomal aberrations are difficult to study in cancer, and therefore several model systems have been developed in recent years. We show that human cells with extra chromosome engineered via microcell-mediated chromosome transfer often gain massive chromosomal rearrangements. The rearrangements arose by chromosome shattering and rejoining as well as by replication-dependent mechanisms. We show that the isolated micronuclei lack functional lamin B1 and become prone to envelope rupture, which leads to DNA damage and aberrant replication. The presence of functional lamin B1 partly correlates with micronuclei size, suggesting that the proper assembly of nuclear envelope might be sensitive to membrane curvature. The chromosomal rearrangements in trisomic cells provide growth advantage compared to cells without rearrangements. Our model system enables to study mechanisms of massive chromosomal rearrangements of any chromosome and their consequences in human cells.

Details

show
hide
Language(s): eng - English
 Dates: 2019
 Publication Status: Published online
 Pages: 20
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000502986200001
DOI: 10.7554/eLife.50292
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: ELIFE
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND : ELIFE SCIENCES PUBLICATIONS LTD
Pages: - Volume / Issue: 8 Sequence Number: e50292 Start / End Page: - Identifier: ISSN: 2050-084X