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  Chloroplast nucleoids are highly dynamic in ploidy, number, and structure during angiosperm leaf development

Greiner, S., Golczyk, H., Malinova, I., Pellizzer, T., Bock, R., Börner, T., et al. (in press). Chloroplast nucleoids are highly dynamic in ploidy, number, and structure during angiosperm leaf development. The Plant Journal, 102(4), 730-746. doi:10.1111/tpj.14658.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-6C56-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-6D9B-2
Genre: Journal Article

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 Creators:
Greiner, S.1, Author              
Golczyk, Hieronim2, Author
Malinova, I.1, Author              
Pellizzer, T.1, Author              
Bock, R.3, Author              
Börner, Thomas2, Author
Herrmann, Reinhold G.2, Author
Affiliations:
1Cytoplasmic and Evolutionary Genetics, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753324              
2External Organizations, ou_persistent22              
3Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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Free keywords: Organelle biogenesis and division, leaf growth and form, Arabidopsis thaliana, Zea mays, Nicotiana tabacum, Beta vulgaris
 Abstract: Abstract Chloroplast nucleoids are large, compact nucleoprotein structures containing multiple copies of the plastid genome. Studies on structural and quantitative changes of plastid DNA (ptDNA) during leaf development are scarce and have produced controversial data. We have systematically investigated nucleoid dynamics and ptDNA quantities in mesophyll of Arabidopsis, tobacco, sugar beet, and maize from the early post-meristematic stage until necrosis. DNA of individual nucleoids was quantified by DAPI-based supersensitive epifluorescence microscopy. Nucleoids occurred in scattered, stacked or ring-shaped arrangements and in recurring patterns during leaf development remarkably similar between the species studied. Nucleoids per organelle varied from few in meristematic plastids to> 30 in mature chloroplasts (corresponding to about 20-750 nucleoids per cell). Nucleoid ploidies ranged from haploid to> 20-fold even within individual organelles, with average values between 2.6- and 6.7-fold and little changes during leaf development. DNA quantities per organelle increased gradually from about a dozen plastome copies in tiny plastids of apex cells to 70-130 copies in chloroplasts of about 7 μm diameter in mature mesophyll tissue, and from about 80 plastome copies in meristematic cells to 2,600-3,300 copies in mature diploid mesophyll cells without conspicuous decline during leaf development. Pulsed-field electrophoresis, restriction of high-molecular weight DNA from chloroplasts and gerontoplasts, and CsCl equilibrium centrifugation of single- and double-stranded ptDNA revealed no noticeable fragmentation of the organelle DNA during leaf development, implying that plastid genomes in mesophyll tissues are remarkably stable until senescence.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Accepted / In Press
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1111/tpj.14658
BibTex Citekey: doi:10.1111/tpj.14658
 Degree: -

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Title: The Plant Journal
  Other : Plant J.
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: 102 (4) Sequence Number: - Start / End Page: 730 - 746 Identifier: ISSN: 0960-7412
CoNE: https://pure.mpg.de/cone/journals/resource/954925579095_1