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  Bioluminescent imaging of single bacterial cells using an enhanced ilux operon.

Gregor, C. (2020). Bioluminescent imaging of single bacterial cells using an enhanced ilux operon. In S. Ripp (Ed.), Bioluminescent Imaging. New York: Humana Pr. doi:10.1007/978-1-4939-9940-8_4.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-6F09-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-9F09-E
Genre: Book Chapter

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3185262.pdf (Publisher version), 833KB
 
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 Creators:
Gregor, C.1, Author              
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1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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Free keywords: Bacteria; Bioluminescence; Imaging; Luciferase; Microscopy; lux
 Abstract: The lux operon is a useful reporter for bioluminescence imaging due to its independence of exogenous luciferin supply, but its relatively low brightness hampers the imaging of single cells. This chapter describes a procedure for the imaging of individual Escherichia coli cells using an improved ilux operon. The enhanced brightness of ilux enables long-term bioluminescence imaging of single bacteria with high sensitivity without the requirement for an external luciferin.

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Language(s): eng - English
 Dates: 2019-11-132020
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1007/978-1-4939-9940-8_4
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Title: Bioluminescent Imaging
Source Genre: Book
 Creator(s):
Ripp, S., Editor
Affiliations:
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Publ. Info: New York : Humana Pr.
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: -

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Title: Methods in Molecular Biology
Source Genre: Series
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Publ. Info: New York : Humana Pr.
Pages: - Volume / Issue: 2081 Sequence Number: - Start / End Page: 43 - 52 Identifier: -