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  Forces during cellular uptake of viruses and nanoparticles at the ventral side

Wiegand, T., Fratini, M., Frey, F., Yserentant, K., Liu, Y., Weber, E., et al. (2020). Forces during cellular uptake of viruses and nanoparticles at the ventral side. Nature Communications, 11(1): 32, pp. 1-13. doi:10.1038/s41467-019-13877-w.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-6FD2-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-0377-1
Genre: Journal Article

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 Creators:
Wiegand, Tina1, 2, Author              
Fratini, Marta1, Author              
Frey, Felix, Author
Yserentant, Klaus, Author
Liu, Yang, Author
Weber, Eva3, Author              
Galior, Kornelia, Author
Ohmes, Julia3, Author              
Braun, Felix, Author
Herten, Dirk-Peter, Author
Boulant, Steeve, Author
Schwarz, Ulrich S., Author
Salaita, Khalid, Author
Cavalcanti-Adam, Elisabetta Ada1, 2, Author              
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, Uniersity of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              
3Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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 Abstract: Many intracellular pathogens, such as mammalian reovirus, mimic extracellular matrix motifs to specifically interact with the host membrane. Whether and how cell-matrix interactions influence virus particle uptake is unknown, as it is usually studied from the dorsal side. Here we show that the forces exerted at the ventral side of adherent cells during reovirus uptake exceed the binding strength of biotin-neutravidin anchoring viruses to a biofunctionalized substrate. Analysis of virus dissociation kinetics using the Bell model revealed mean forces higher than 30 pN per virus, preferentially applied in the cell periphery where close matrix contacts form. Utilizing 100 nm-sized nanoparticles decorated with integrin adhesion motifs, we demonstrate that the uptake forces scale with the adhesion energy, while actin/myosin inhibitions strongly reduce the uptake frequency, but not uptake kinetics. We hypothesize that particle adhesion and the push by the substrate provide the main driving forces for uptake.

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Language(s): eng - English
 Dates: 2018-12-112019-12-062020-01-02
 Publication Status: Published online
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 11 (1) Sequence Number: 32 Start / End Page: 1 - 13 Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723