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  Low-signal limit of X-ray single particle diffractive imaging

Ayyer, K., Morgan, A. J., Aquila, A., DeMirci, H., Hogue, B. G., Kirian, R. A., et al. (2019). Low-signal limit of X-ray single particle diffractive imaging. Optics Express, 27(26), 37816-37833. doi:10.1364/OE.27.037816.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-6FF0-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-17AE-C
Genre: Journal Article

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oe-27-26-37816.pdf (Publisher version), 961KB
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oe-27-26-37816.pdf
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Open Access – Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
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2019
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© The Optical Society / the Author(s)

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https://dx.doi.org/10.1364/OE.27.037816 (Publisher version)
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 Creators:
Ayyer, K.1, 2, Author              
Morgan, A. J.2, Author
Aquila, A.3, Author
DeMirci, H.4, 5, 6, Author
Hogue, B. G.7, 8, 9, Author
Kirian, R. A.10, Author
Paulraj, L. X.2, 3, Author
Yoon, C. H.3, Author
Chapman, H. N.2, 11, 12, Author
Barty, A.2, Author
Affiliations:
1Computational Nanoscale Imaging, Condensed Matter Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_3012829              
2Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron DESY, ou_persistent22              
3Linac Coherent Light Source, SLAC National Accelerator Laboratory, ou_persistent22              
4Biosciences Division, SLAC National Accelerator Laboratory, ou_persistent22              
5Stanford PULSE Institute, SLAC National Accelerator Laboratory, ou_persistent22              
6Department of Molecular Biology and Genetics, Koc University, ou_persistent22              
7Biodesign Center for Immunotherapy, Vaccines, and Virotherapy, Biodesign Institute at Arizona State University, ou_persistent22              
8iodesign Center for Applied Structural Discovery, Biodesign Institute at Arizona State University, ou_persistent22              
9Arizona State University, School of Life Sciences (SOLS), ou_persistent22              
10Department of Physics, Arizona State University, ou_persistent22              
11Department of Physics, Universität Hamburg, ou_persistent22              
12The Hamburg Center for Ultrafast Imaging, Universität Hamburg, ou_persistent22              

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 Abstract: An outstanding question in X-ray single particle imaging experiments has been the feasibility of imaging sub 10-nm-sized biomolecules under realistic experimental conditions where very few photons are expected to be measured in a single snapshot and instrument background may be significant relative to particle scattering. While analyses of simulated data have shown that the determination of an average image should be feasible using Bayesian methods such as the EMC algorithm, this has yet to be demonstrated using experimental data containing realistic non-isotropic instrument background, sample variability and other experimental factors. In this work, we show that the orientation and phase retrieval steps work at photon counts diluted to the signal levels one expects from smaller molecules or with weaker pulses, using data from experimental measurements of 60-nm PR772 viruses. Even when the signal is reduced to a fraction as little as 1/256, the virus electron density determined using ab initio phasing is of almost the same quality as the high-signal data. However, we are still limited by the total number of patterns collected, which may soon be mitigated by the advent of high repetition-rate sources like the European XFEL and LCLS-II.

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Language(s): eng - English
 Dates: 2019-11-212019-10-252019-11-252019-12-122019-12-23
 Publication Status: Published in print
 Pages: 18
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1364/OE.27.037816
 Degree: -

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Title: Optics Express
  Abbreviation : Opt. Express
Source Genre: Journal
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Publ. Info: Washington, DC : Optical Society of America
Pages: - Volume / Issue: 27 (26) Sequence Number: - Start / End Page: 37816 - 37833 Identifier: ISSN: 1094-4087
CoNE: https://pure.mpg.de/cone/journals/resource/954925609918