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  Follicle-stimulating hormone and luteinizing hormone increase Ca2+ in the granulosa cells of mouse ovarian follicles

Egbert, J. R., Fahey, P. G., Reimer, J., Owen, C. M., Evsikov, A. V., Nikolaev, V. O., et al. (2019). Follicle-stimulating hormone and luteinizing hormone increase Ca2+ in the granulosa cells of mouse ovarian follicles. Biology of Reproduction, 101(2), 433-444. doi:10.1093/biolre/ioz085.

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 Creators:
Egbert, Jeremy R., Author
Fahey, Paul G., Author
Reimer, Jacob, Author
Owen, Corie M., Author
Evsikov, Alexei V., Author
Nikolaev, Viacheslav O., Author
Griesbeck, Oliver1, Author           
Ray, Russell S., Author
Tolias, Andreas S., Author
Jaffe, Laurinda A., Author
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1Research Group: Tools for Bio-Imaging / Griesbeck, MPI of Neurobiology, Max Planck Society, ou_1113560              

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Free keywords: OOCYTE MATURATION; CALCIUM-ION; MECHANISM; RECEPTOR; MEIOSIS; SENSORS; PROTEIN; KINASE; COMMUNICATION; MOBILIZATIONReproductive Biology; calcium; follicle; follicle-stimulating hormone; gonadotropins; granulosa cells; luteinizing hormone;
 Abstract: In mammalian ovarian follicles, follicle stimulating hormone (FSH) and luteinizing hormone (LH) signal primarily through the G-protein G(s) to elevate cAMP, but both of these hormones can also elevate Ca2+ under some conditions. Here, we investigate FSH- and LH-induced Ca2+ signaling in intact follicles of mice expressing genetically encoded Ca2+ sensors, Twitch-2B and GCaMP6s. At a physiological concentration (1 nM), FSH elevates Ca2+ within the granulosa cells of preantral and antral follicles. The Ca2+ rise begins several minutes after FSH application, peaks at similar to 10 min, remains above baseline for another similar to 10 min, and depends on extracellular Ca2+. However, suppression of the FSH-induced Ca2+ increase by reducing extracellular Ca2+ does not inhibit FSH-induced phosphorylation ofMAP kinase, estradiol production, or the acquisition of LH responsiveness. Like FSH, LH also increases Ca2+, when applied to preovulatory follicles. At a physiological concentration (10 nM), LH elicits Ca2+ oscillations in a subset of cells in the outer mural granulosa layer. These oscillations continue for at least 6 h and depend on the activity of G(q) family G-proteins. Suppression of the oscillations by G(q) inhibition does not inhibit meiotic resumption, but does delay the time to 50% ovulation by about 3 h. In summary, both FSH and LH increase Ca2+ in the granulosa cells of intact follicles, but the functions of these Ca2+ rises are only starting to be identified. Summary Sentence Both FSH and LH increase Ca2+ in the granulosa cells of intact ovarian follicles from mice expressing genetically encoded sensors.

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Language(s): eng - English
 Dates: 2019-08-01
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000492976600018
DOI: 10.1093/biolre/ioz085
 Degree: -

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Title: Biology of Reproduction
  Other : Biol. Reprod.
Source Genre: Journal
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Publ. Info: Champaign, Ill. : Society for the Study of Reproduction
Pages: - Volume / Issue: 101 (2) Sequence Number: - Start / End Page: 433 - 444 Identifier: ISSN: 0006-3363
CoNE: https://pure.mpg.de/cone/journals/resource/954925385114