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  Dynamic tuning of FRET in a green fluorescent protein biosensor

Trigo-Mourino, P., Thestrup, T., Griesbeck, O., Griesinger, C., & Becker, S. (2019). Dynamic tuning of FRET in a green fluorescent protein biosensor. Science Advances, 5(8): eaaw4988. doi:10.1126/sciadv.aaw4988.

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eaaw4988.full.pdf (Publisher version), 2MB
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© 2019 The Authors

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 Creators:
Trigo-Mourino, Pablo, Author
Thestrup, Thomas1, Author              
Griesbeck, Oliver1, Author              
Griesinger, Christian, Author
Becker, Stefan, Author
Affiliations:
1Research Group: Tools for Bio-Imaging / Griesbeck, MPI of Neurobiology, Max Planck Society, ou_1113560              

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Free keywords: RESONANCE ENERGY-TRANSFER; RAY SOLUTION SCATTERING; SYSTEM; MACROMOLECULES; REFINEMENT; INDICATORS; CA2+Science & Technology - Other Topics;
 Abstract: Forster resonance energy transfer (FRET) between mutants of green fluorescent protein is widely used to monitor protein-protein interactions and as a readout mode in fluorescent biosensors. Despite the fundamental importance of distance and molecular angles of fluorophores to each other, structural details on fluorescent protein FRET have been missing. Here, we report the high-resolution x-ray structure of the fluorescent proteins mCerulean3 and cpVenus within the biosensor Twitch-2B, as they undergo FRET and characterize the dynamics of this biosensor with B-0(2)-dependent paramagnetic nuclear magnetic resonance at 900 MHz and 1.1 GHz. These structural data provide the unprecedented opportunity to calculate FRET from the x-ray structure and to compare it to experimental data in solution. We find that interdomain dynamics limits the FRET effect and show that a rigidification of the sensor further enhances FRET.

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Language(s): eng - English
 Dates: 2019-08-07
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000481798400031
DOI: 10.1126/sciadv.aaw4988
 Degree: -

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Title: Science Advances
  Other : Sci. Adv.
Source Genre: Journal
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Publ. Info: Washington : AAAS
Pages: - Volume / Issue: 5 (8) Sequence Number: eaaw4988 Start / End Page: - Identifier: ISSN: 2375-2548
CoNE: https://pure.mpg.de/cone/journals/resource/2375-2548