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  Analysis of cell numbers in immunohistochemically stained brain sections using a computerized image analysis system

Benali, A., Leefken, I., Eysel, U., & Weiler, E. (2003). Analysis of cell numbers in immunohistochemically stained brain sections using a computerized image analysis system. Poster presented at 5th Meeting of the German Neuroscience Society, 29th Göttingen Neurobiology Conference, Göttingen, Germany.

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 Creators:
Benali, A, Author
Leefken, I, Author
Eysel, UT, Author
Weiler, E1, Author              
Affiliations:
1External Organizations, ou_persistent22              

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 Abstract: Neuronal networks are established by cells with different characteristics, for example the ability to produce specific transmitters or to buffer calcium-ions by expressing calcium-binding proteins. To understand neuronal networks, it is essential to know, how many cells express the specific markers, which can be visualized immunohistochemically. Cells numbers then have to be evaluated. Here we propose a reliable method for automatic counting of cells in 5 \(\mu\)m paraffin sections of the brain, labelled with different antibodies (against NeuN, parvalbumin, GABA and GAD) or stained with Nissl. Images of stained sections are acquired with a CCD-camera on the microscope and are converted to binary images by thresholding. Clusters of “ON pixels” (value of 1) corresponding to cell bodies are selected based on size. The parameters of the algorithm (intensity range and cluster-size) are adjusted for different methods of staining according to expert knowledge. Cell size is simultaneously determined by the histogram. The presented automatic cell counting method (ACCM) provides correct counting results as demonstrated by a comparison of computational results with counts gained by human experimenters and with a commercially available image analysis system. On the basis of ACCM counts small and perhaps physiologically relevant differences in the number of labelled cells can be revealed, as demonstrated here for the GABAergic system after two hours of an electrical stimulation, changing neuronal network characteristics.

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 Dates: 2003-06
 Publication Status: Published in print
 Pages: -
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Title: 5th Meeting of the German Neuroscience Society, 29th Göttingen Neurobiology Conference
Place of Event: Göttingen, Germany
Start-/End Date: 2003-06-11 - 2003-06-15

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Title: The Neurosciences from Basic Research to Therapy: Proceedings of the 29th Göttingen Neurobiology Conference and the 5th Meeting of the German Neuroscience Society 2003
Source Genre: Proceedings
 Creator(s):
Elsner, N, Editor
Zimmermann, H, Editor
Affiliations:
-
Publ. Info: Stuttgart, Germany : Thieme
Pages: - Volume / Issue: - Sequence Number: 695 Start / End Page: 740 - 741 Identifier: ISBN: 3-13-137351-2