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  Active role of elongation factor G in maintaining the mRNA reading frame during translation.

Peng, B. Z., Bock, L. V., Belardinelli, R., Peske, F., Grubmüller, H., & Rodnina, M. V. (2019). Active role of elongation factor G in maintaining the mRNA reading frame during translation. Science Advances, 5(12): eaax8030. doi:10.1126/sciadv.aax8030.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-7983-F Version Permalink: http://hdl.handle.net/21.11116/0000-0005-7987-B
Genre: Journal Article

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 Creators:
Peng, B. Z.1, Author              
Bock, L. V.2, Author              
Belardinelli, R.1, Author              
Peske, F.1, Author              
Grubmüller, H.2, Author              
Rodnina, M. V.1, Author              
Affiliations:
1Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              
2Department of Theoretical and Computational Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578631              

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 Abstract: During translation, the ribosome moves along the mRNA one codon at a time with the help of elongation factor G (EF-G). Spontaneous changes in the translational reading frame are extremely rare, yet how the precise triplet-wise step is maintained is not clear. Here, we show that the ribosome is prone to spontaneous frameshifting on mRNA slippery sequences, whereas EF-G restricts frameshifting. EF-G helps to maintain the mRNA reading frame by guiding the A-site transfer RNA during translocation due to specific interactions with the tip of EF-G domain 4. Furthermore, EF-G accelerates ribosome rearrangements that restore the ribosome's control over the codon-anticodon interaction at the end of the movement. Our data explain how the mRNA reading frame is maintained during translation.

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Language(s): eng - English
 Dates: 2019-12-042019-12-18
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1126/sciadv.aax8030
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Title: Science Advances
Source Genre: Journal
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Pages: 9 Volume / Issue: 5 (12) Sequence Number: eaax8030 Start / End Page: - Identifier: -