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  Direct Visualization of Single Nuclear Pore Complex Proteins Using Genetically-Encoded Probes for DNA-PAINT

Schlichthaerle, T., Strauss, M. T., Schueder, F., Auer, A., Nijmeijer, B., Kueblbeck, M., et al. (2019). Direct Visualization of Single Nuclear Pore Complex Proteins Using Genetically-Encoded Probes for DNA-PAINT. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 58(37, SI), 13004-13008. doi:10.1002/anie.201905685.

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Schlichthaerle_et_al-2019-Angewandte_Chemie_International_Edition.pdf (Publisher version), 7MB
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Schlichthaerle_et_al-2019-Angewandte_Chemie_International_Edition.pdf
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 Creators:
Schlichthaerle, Thomas1, Author              
Strauss, Maximilian T.1, Author              
Schueder, Florian1, Author              
Auer, Alexander1, Author              
Nijmeijer, Bianca2, Author
Kueblbeck, Moritz2, Author
Sabinina, Vilma Jimenez2, Author
Thevathasan V, Jervis2, Author
Ries, Jonas2, Author
Ellenberg, Jan2, Author
Jungmann, Ralf1, Author              
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              
2external, ou_persistent22              

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Free keywords: SUPERRESOLUTION MICROSCOPY; MOLECULES; SCAFFOLD; DESIGNChemistry; DNA-PAINT; genetically encoded tags; nuclear pore complex; single-molecule imaging; super-resolution microscopy;
 Abstract: The nuclear pore complex (NPC) is one of the largest and most complex protein assemblies in the cell and, among other functions, serves as the gatekeeper of nucleocytoplasmic transport. Unraveling its molecular architecture and functioning has been an active research topic for decades with recent cryogenic electron microscopy and super-resolution studies advancing our understanding of the architecture of the NPC complex. However, the specific and direct visualization of single copies of NPC proteins is thus far elusive. Herein, we combine genetically-encoded self-labeling enzymes such as SNAP-tag and HaloTag with DNA-PAINT microscopy. We resolve single copies of nucleoporins in the human Y-complex in three dimensions with a precision of circa 3 nm, enabling studies of multicomponent complexes on the level of single proteins in cells using optical fluorescence microscopy.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000482401000001
DOI: 10.1002/anie.201905685
 Degree: -

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Title: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
  Alternative Title : Special Issue: Science Forum 2019
Source Genre: Journal
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Publ. Info: POSTFACH 101161, 69451 WEINHEIM, GERMANY : WILEY-V C H VERLAG GMBH
Pages: - Volume / Issue: 58 (37, SI) Sequence Number: - Start / End Page: 13004 - 13008 Identifier: ISSN: 1433-7851