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  F-actin interactome reveals vimentin as a key regulator of actin organization and cell mechanics in mitosis.

Serres, M. P., Samwer, M., Truong Quang, B. A., Lavoie, G., Perera, U., Görlich, D., et al. (2020). F-actin interactome reveals vimentin as a key regulator of actin organization and cell mechanics in mitosis. Developmental Cell, 52(2), 210-222. doi:10.1016/j.devcel.2019.12.011.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-7B59-E Version Permalink: http://hdl.handle.net/21.11116/0000-0005-939E-3
Genre: Journal Article

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 Creators:
Serres, M. P., Author
Samwer, M.1, Author              
Truong Quang, B. A., Author
Lavoie, G., Author
Perera, U., Author
Görlich, D.2, Author              
Charras, G., Author
Petronczki, M., Author
Roux, P. P., Author
Paluch, E. K., Author
Affiliations:
1Department of Cellular Logistics, MPI for Biophysical Chemistry, Max Planck Society, ou_578574              
2Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society, ou_578574              

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Free keywords: CDK1; actin organization; cell shape; cortex; mitosis; mitotic rounding; phosphorylation; plectin; proteomics; vimentin
 Abstract: Most metazoan cells entering mitosis undergo characteristic rounding, which is important for accurate spindle positioning and chromosome separation. Rounding is driven by contractile tension generated by myosin motors in the sub-membranous actin cortex. Recent studies highlight that alongside myosin activity, cortical actin organization is a key regulator of cortex tension. Yet, how mitotic actin organization is controlled remains poorly understood. To address this, we characterized the F-actin interactome in spread interphase and round mitotic cells. Using super-resolution microscopy, we then screened for regulators of cortex architecture and identified the intermediate filament vimentin and the actin-vimentin linker plectin as unexpected candidates. We found that vimentin is recruited to the mitotic cortex in a plectin-dependent manner. We then showed that cortical vimentin controls actin network organization and mechanics in mitosis and is required for successful cell division in confinement. Together, our study highlights crucial interactions between cytoskeletal networks during cell division.

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Language(s): eng - English
 Dates: 2020-01-092020-01-27
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.devcel.2019.12.011
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Title: Developmental Cell
Source Genre: Journal
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Pages: - Volume / Issue: 52 (2) Sequence Number: - Start / End Page: 210 - 222 Identifier: -