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  Reversible pH-responsive coacervate formation in lipid vesicles activates dormant enzymatic reactions

Love, C., Steinkühler, J., Gonzales, D. T., Yandrapalli, N., Robinson, T., Dimova, R., et al. (2020). Reversible pH-responsive coacervate formation in lipid vesicles activates dormant enzymatic reactions. Angewandte Chemie, International Edition, 59(15), 5950-5957. doi:10.1002/anie.201914893.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0005-8DB8-D Version Permalink: http://hdl.handle.net/21.11116/0000-0006-B3A1-9
Genre: Journal Article

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 Creators:
Love, Celina, Author
Steinkühler, Jan1, Author              
Gonzales, David T., Author
Yandrapalli, Naresh2, Author              
Robinson, Tom2, Author              
Dimova, Rumiana1, Author              
Tang, T.-Y. Dora, Author
Affiliations:
1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              
2Tom Robinson, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2288691              

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Free keywords: Liquid-liquid phase separation, dynamic hybrid protocells, microfluidics, Coacervates, lipid vesicles
 Abstract: In situ, reversible coacervate formation within lipid vesicles represents a key step in the development of responsive synthetic cellular models. Here we exploit the pH responsiveness of a polycation above and below its pKa, to drive liquid-liquid phase separation to form single coacervate droplets within lipid vesicles.  The process is completely reversible as coacervate droplets can be disassembled by increasing the pH above the pKa. We further show that pH triggered coacervation in the presence of low concentrations of enzymes activate dormant enzyme reactions by molecular up-concentration into the coacervate droplets. In conclusion, this work establishes a tuneable, pH responsive, enzymatically active multi-compartment synthetic cell. The system is readily transferred into microfluidics, making it a robust model for addressing general questions in biology such as the role of phase separation and its effect on enzymatic reactions using a bottom-up synthetic biology approach.
 Abstract: Die pH‐Responsivität eines Polykations über und unter seinem pKa wurde genutzt, um eine Flüssig‐flüssig‐Phasentrennung unter Bildung von Koazervattröpfchen innerhalb von Lipidvesikeln anzutreiben. Dies erfolgt reversibel, da die Tröpfchen durch Erhöhen des pH über den pKa aufgelöst werden können. pH‐induzierte Koazervierung in Gegenwart niedriger Enzymkonzentrationen aktiviert ruhende Enzymreaktionen, da die lokalen Enzym‐ und Substratkonzentrationen in den Tröpfchen erhöht sind.

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Language(s): eng - English
 Dates: 2020-01-142020
 Publication Status: Published in print
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Title: Angewandte Chemie, International Edition
  Abbreviation : Angew. Chem., Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 59 (15) Sequence Number: - Start / End Page: 5950 - 5957 Identifier: ISSN: 1433-7851

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Title: Angewandte Chemie
  Abbreviation : Angew. Chem.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 132 (15) Sequence Number: - Start / End Page: 6006 - 6013 Identifier: ISSN: 0044-8249