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  Transient DNA Occupancy of the SMC Interarm Space in Prokaryotic Condensin

Vasquez Nunez, R., Ruiz Avila, L. B., & Gruber, S. (2019). Transient DNA Occupancy of the SMC Interarm Space in Prokaryotic Condensin. MOLECULAR CELL, 75(2), 209-223.e6. doi:10.1016/j.molcel.2019.05.001.

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Genre: Journal Article

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 Creators:
Vasquez Nunez, Roberto1, Author
Ruiz Avila, Laura B.2, Author           
Gruber, Stephan2, Author           
Affiliations:
1external, ou_persistent22              
2Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565151              

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Free keywords: PROMOTES CHROMOSOME SEGREGATION; STRUCTURAL MAINTENANCE; MOLECULAR-BASIS; COMPLEX; ATP; BINDING; ACTIVATION; ORIGIN; ARMS; ORGANIZATIONBiochemistry & Molecular Biology; Cell Biology;
 Abstract: Multi-subunit SMC ATPases control chromosome superstructure and DNA topology, presumably by DNA translocation and loop extrusion. Chromosomal DNA is entrapped within the tripartite SMCkleisin ring. Juxtaposed SMC heads ("J heads") or engaged SMC heads ("E heads") split the SMCkleisin ring into "S" and "K" sub-compartments. Here, we map a DNA-binding interface to the S compartment of E heads SmcScpAB and show that head-DNA association is essential for efficient DNA translocation and for traversing highly transcribed genes in Bacillus subtilis. We demonstrate that in J heads, SmcScpAB chromosomal DNA resides in the K compartment but is absent from the S compartment. Our results imply that the DNA occupancy of the S compartment changes during the ATP hydrolysis cycle. We propose that DNA translocation involves DNA entry into and exit out of the S compartment, possibly by DNA transfer between compartments and DNA segment capture.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 21
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: MOLECULAR CELL
Source Genre: Journal
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Publ. Info: 50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 75 (2) Sequence Number: - Start / End Page: 209 - 223.e6 Identifier: ISSN: 1097-2765