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  Atg11 tethers Atg9 vesicles to initiate selective autophagy

Matscheko, N., Mayrhofer, P., Rao, Y., Beier, V., & Wollert, T. (2019). Atg11 tethers Atg9 vesicles to initiate selective autophagy. PLoS Biology, 17(7): e3000377. doi:10.1371/journal.pbio.3000377.

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 Creators:
Matscheko, Nena1, Author              
Mayrhofer, Peter2, Author
Rao, Yijian1, Author              
Beier, Viola1, Author              
Wollert, Thomas2, Author
Affiliations:
1Wollert, Thomas / Molecular Membrane and Organelle Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565175              
2external, ou_persistent22              

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Free keywords: SCAFFOLD PROTEIN; CARGO; YEAST; MECHANISM; CYTOPLASM; PHOSPHORYLATION; SEDIMENTATION; DEGRADATION; MACHINERY; MITOPHAGYBiochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics;
 Abstract: Autophagy recycles cytoplasmic components by sequestering them in double membrane-surrounded autophagosomes. The two proteins Atg11 and Atg17 are scaffolding components of the Atg1 kinase complex. Atg17 recruits and tethers Atg9-donor vesicles, and the corresponding Atg1 kinase complex induces the formation of nonselective autophagosomes. Atg11 initiates selective autophagy and coordinates the switch to nonselective autophagy by recruiting Atg17. The molecular function of Atg11 remained, however, less well understood. Here, we demonstrate that Atg11 is activated by cargo through a direct interaction with autophagy receptors. Activated Atg11 dimerizes and tethers Atg9 vesicles, which leads to the nucleation of phagophores in direct vicinity of cargo. Starvation reciprocally regulates the activity of both tethering factors by initiating the degradation of Atg11 while Atg17 is activated. This allows Atg17 to sequester and tether Atg9 vesicles independent of cargo to nucleate nonselective phagophores. Our data reveal insights into the molecular mechanisms governing cargo selection and specificity in autophagy.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published online
 Pages: 21
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: PLoS Biology
  Other : PLoS Biol.
Source Genre: Journal
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Publ. Info: San Francisco, California, US : Public Library of Science
Pages: - Volume / Issue: 17 (7) Sequence Number: e3000377 Start / End Page: - Identifier: ISSN: 1544-9173
CoNE: https://pure.mpg.de/cone/journals/resource/111056649444170