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  VIPP1 rods engulf membranes containing phosphatidylinositol phosphates

Theis, J., Gupta, T. K., Klingler, J., Wan, W., Albert, S., Keller, S., et al. (2019). VIPP1 rods engulf membranes containing phosphatidylinositol phosphates. SCIENTIFIC REPORTS, 9: 8725. doi:10.1038/s41598-019-44259-3.

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 Creators:
Theis, Jasmine1, Author
Gupta, Tilak Kumar2, Author              
Klingler, Johannes1, Author
Wan, William2, Author              
Albert, Sahradha2, Author              
Keller, Sandro1, Author
Engel, Benjamin D.2, Author              
Schroda, Michael1, Author
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1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: VESICLE-INDUCING PROTEIN; MICROTUBULE-LIKE STRUCTURES; FINE-STRUCTURE; PHAGE SHOCK; GRPE HOMOLOG; PLASTIDS 1; SYNECHOCYSTIS; ENVELOPE; PSPA; DOMAINScience & Technology - Other Topics;
 Abstract: In cyanobacteria and plants, VIPP1 plays crucial roles in the biogenesis and repair of thylakoid membrane protein complexes and in coping with chloroplast membrane stress. In chloroplasts, VIPP1 localizes in distinct patterns at or close to envelope and thylakoid membranes. In vitro, VIPP1 forms higher-order oligomers of >1 MDa that organize into rings and rods. However, it remains unknown how VIPP1 oligomerization is related to function. Using time-resolved fluorescence anisotropy and sucrose density gradient centrifugation, we show here that Chlamydomonas reinhardtiiVIPP1 binds strongly to liposomal membranes containing phosphatidylinositol-4-phosphate (PI4P). Cryo-electron tomography reveals that VIPP1 oligomerizes into rods that can engulf liposomal membranes containing PI4P. These findings place VIPP1 into a group of membrane-shaping proteins including epsin and BAR domain proteins. Moreover, they point to a potential role of phosphatidylinositols in directing the shaping of chloroplast membranes.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: SCIENTIFIC REPORTS
Source Genre: Journal
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Publ. Info: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 9 Sequence Number: 8725 Start / End Page: - Identifier: ISSN: 2045-2322