English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Structural heterogeneity of attC integron recombination sites revealed by optical tweezers

Mukhortava, A., Pöge, M., Grieb, M. S., Nivina, A., Loot, C., Mazel, D., et al. (2019). Structural heterogeneity of attC integron recombination sites revealed by optical tweezers. NUCLEIC ACIDS RESEARCH, 47(4), 1861-1870. doi:10.1093/nar/gky1258.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
gky1258.pdf (Any fulltext), 3MB
Name:
gky1258.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
open access article
License:
-

Locators

show

Creators

show
hide
 Creators:
Mukhortava, Ann1, Author
Pöge, Matthias2, Author              
Grieb, Maj Svea1, Author
Nivina, Aleksandra1, Author
Loot, Celine1, Author
Mazel, Didier1, Author
Schlierf, Michael1, Author
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

Content

show
hide
Free keywords: SINGLE-STRANDED-DNA; CASSETTE INSERTION; ELASTIC PROPERTIES; MOLECULES; BINDSBiochemistry & Molecular Biology;
 Abstract: A predominant tool for adaptation in Gram-negative bacteria is the functional genetic platform called integron. Integrons capture and rearrange promoterless gene cassettes in a unique recombination process involving the recognition of folded single-stranded DNA hairpinsso-called attC siteswith a strong preference for the attC bottom strand. While structural elements have been identified to promote this preference, their mechanistic action remains incomplete. Here, we used high-resolution single-molecule optical tweezers (OT) to characterize secondary structures formed by the attC bottom () and top () strands of the paradigmatic attC(aadA7) site. We found for both sequences two structuresa straight, canonical hairpin and a kinked hairpin. Remarkably, the recombination-preferred predominantly formed the straight hairpin, while the preferentially adopted the kinked structure, which exposes only one complete recombinase binding box. By a mutational analysis, we identified three bases in the unpaired central spacer, which could invert the preferred conformations and increase the recombination frequency of the in vivo. A bioinformatics screen revealed structural bias toward a straight, canonical hairpin conformation in the bottom strand of many antibiotic resistance cassettes attC sites. Thus, we anticipate that structural fine tuning could be a mechanism in many biologically active DNA hairpins.

Details

show
hide
Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000467961200025
DOI: 10.1093/nar/gky1258
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: NUCLEIC ACIDS RESEARCH
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND : OXFORD UNIV PRESS
Pages: - Volume / Issue: 47 (4) Sequence Number: - Start / End Page: 1861 - 1870 Identifier: ISSN: 0305-1048